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Fig. S1 Localization of α-tomatine and rutin in tomato.

Fig. S2 Detoxification of dehydrotomatine by Cladosporium fulvum.

Fig. S3 In vitro detoxification of α-tomatine by Cladosporium fulvum.

Fig. S4 Southern blot analysis of the ∆cftom1 mutants.

Fig. S5 Effects of α-tomatine and tomatidine on H2O2 accumulation in chitin-treated tomato MSK8 cell suspensions.

Table S1 Oligonucleotides used in this study

Table S2 Accurate mass LC-MS and MS/MS data of the major tomato alkaloids affected by CfTom1 enzyme