Article first published online: 20 MAR 2013
© 2013 The Author. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 2, pages 325–327, April 2013
How to Cite
Franks, P. J. (2013), Passive and active stomatal control: either or both?. New Phytologist, 198: 325–327. doi: 10.1111/nph.12228
- Issue published online: 20 MAR 2013
- Article first published online: 20 MAR 2013
- abscisic acid (ABA);
- hydropassive and hydroactive;
- leaf water status;
- stomatal conductance;
More than a century ago, Francis Darwin (1898) combined two key observations that shape current understanding of the role of leaf water status in stomatal control: that ‘the guard cells share in the general turgor of the leaf’, and that ‘guard cells may lose turgor spontaneously, …in response to a stimulus [that] may be the slight flaccidity of the rest of the leaf’. The principle of this dual action was rejected by others at the time but is evident in the data of Knight (1917) (Fig. 1), and the two components later became known respectively as ‘hydropassive’ and ‘hydroactive’ stomatal responses to leaf water deficit (Stålfelt, 1929, 1955; Raschke, 1970; Cowan, 1977). Now the discussion around this subject has been re-invigorated from a fresh perspective, one barely accessible to Francis Darwin and his contemporaries: evolution. Focusing on stomatal behaviour in ferns and lycophytes, two basal lineages of vascular plants, McAdam & Brodribb in this issue of New Phytologist (pp. 429–441) report that stomatal closure under water deficit in these plants is dominated by the passive mechanism, with the active component playing a lesser role.
‘Now the discussion around this subject has been re-invigorated from a fresh perspective, one barely accessible to Francis Darwin and his contemporaries: evolution.’
How plants manage water deficit under drought or high evaporative demand profoundly influences primary productivity and survival in diverse environments (Boyer, 1982; Ciais et al., 2005). The stomatal control system is at the centre of this, responding rapidly to small environmental changes to optimize the exchange of water for carbon (Farquhar & Sharkey, 1982). One approach to understanding this system has been to examine the molecular signalling mechanisms by which environmental changes are sensed, integrated and translated into adjustments in stomatal aperture. From this, major inroads have been made into the hydroactive mechanism, with considerable attention to the action of the stress hormone abscisic acid (ABA) (Schroeder et al., 2001; Kim et al., 2010). Since its ability to reduce stomatal aperture was discovered (Mittelheuser & Van Steveninck, 1969), ABA has gained increasing prominence as a key player in stomatal control, particularly in relation to management of plant water deficit. However, almost all of the research into the action of ABA and the hydroactive mechanism has focused on angiosperms. Could this have biased current perceptions of the relative role of the hydroactive and hydropassive mechanisms? The findings of McAdam & Brodribb suggest this could be the case. In a group of six ferns and one lycophyte, only two of the ferns showed the classical pattern of decreasing stomatal conductance with increasing leaf ABA concentration, but all closed their stomata as leaf water potential declined, leading McAdam & Brodribb to conclude that ferns and lycophytes rely predominantly on the hydropassive mechanism.
Given that stomatal sensitivity to ABA and related molecular signalling pathways have been identified across the evolutionary spectrum of vascular plants, as well as in moss sporophytes (Chater et al., 2011; Ruszala et al., 2011), it appears that hydroactive and hydropassive stomatal control processes have operated together since stomata first evolved some 400 million yr ago. The question that emerges is whether angiosperms are generally more dependent on ABA and the hydroactive mechanism, and if so, why? To address this, one needs to consider the conditions that could have driven selection for such dependence. Increasing exposure to leaf water deficit per se is unlikely to have been a significant factor as ferns, lycophytes and angiosperms today thrive in a diverse range of moisture environments. However, selection for other stomatal performance characteristics might have increased the dependence of angiosperms on active adjustment of guard cell turgor. One possibility is illustrated in Fig. 2. In contrast to ferns and lycophytes, the epidermal cells adjacent to stomata in angiosperms typically exert a ‘mechanical advantage’ over the guard cells. This enhances dynamic performance but for stomata to close effectively under declining leaf water potential the mechanical advantage must be counteracted by active guard cell osmotic adjustment (Franks & Farquhar, 2007). ABA might serve as a signal in this mechanism. Importantly, it is shown in Fig. 2 that although the absence of the mechanical advantage (as in some ferns and lycophytes) would obviate the need for this kind of hydroactive mechanism, the improved sensitivity it provides would benefit most plants. The relative importance of hydropassive and hydroactive stomatal control in different plant species or lineages may vary as a result of a number of factors, but so far, Darwin's integrated model appears to be holding up well.
- 1982. Plant productivity and environment. Science 218: 443–448. .
- 2011. Regulatory mechanism controlling stomatal behavior conserved across 400 million years of land plant evolution. Current Biology 21: 1025–1029. , , , , , , .
- 2005. Europe-wide reduction in primary productivity caused by the heat and drought in 2003. Nature 437: 529–533. , , , , , , , , , et al.
- 1977. Stomatal behaviour and environment. Advances in Botanical Research 4: 117–228. .
- 1898. Observations on stomata. Philosophical Transactions of the Royal Society of London Series B 190: 531–621. .
- 1982. Stomatal conductance and photosynthesis. Annual Review of Plant Physiology 33: 17–45. , .
- 2007. The mechanical diversity of stomata and its significance in gas exchange control. Plant Physiology 143: 78–87. , .
- 2010. Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annual Review of Plant Biology 61: 561–591. , , , , .
- 1917. The interrelations of stomatal aperture, leaf water content, and transpiration rate. Annals of Botany 31: 221–240. .
- 2013. Ancestral stomatal control results in a canalization of fern and lycophyte adaptation to drought. New Phytologist 198: 429–441. , .
- 1969. Stomatal closure and inhibition of transpiration induced by (RS)-abscisic acid. Nature 221: 281–282. , .
- 1970. Leaf hydraulic system: rapid epidermal and stomatal responses to changes in water supply. Science 167: 189–191. .
- 2011. Land plants acquired active stomatal control early in their evolutionary history. Current Biology 21: 1030–1035. , , , , , , .
- 2001. Guard cell abscisic acid signalling and engineering drought hardiness in plants. Nature 410: 327–330. , , .
- 1929. Die Abhängigkeit der spaltöffnungsreaktionen von der wasserbilanz. Planta 8: 287–340. .
- 1955. The stomata as a hydrophotic regulator of the water deficit of the plant. Physiologia Plantarum 8: 572–593. .