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nph12233-sup-0001-FigS1-S12.pptapplication/PPT3472K

Fig. S1 Putative DNA-binding motifs identified in the Arabidopsis thaliana PROPEP1 gene promoter by searching various databases.

Fig. S2 The 5′ deletion analyses of the PROPEP1 promoter:GUS constructs in parsley protoplasts.

Fig. S3 Putative DNA-binding motifs identified in the Arabidopsis thaliana PROPEP2 gene promoter by searching various databases.

Fig. S4 Putative DNA-binding motifs identified in the Arabidopsis thaliana PROPEP3 gene promoter by searching various databases.

Fig. S5 GUS activity staining of flagellin22 (flg22)-treated 4-wk-old leaves derived from Arabidopsis thaliana lines carrying different indicated PROPEP2:GUS reporter transgenes.

Fig. S6 Immunoblot detection of PROPEP1-GFP fusion protein in different Arabidopsis thaliana Columbia (Col-0) and pepr1 pepr2 transgenic lines upon flagellin22 (flg22) treatment.

Fig. S7 GUS activity staining of flagellin22 (flg22)-treated 4-wk-old leaves derived from Arabidopsis thaliana lines carrying different indicated PROPEP3:GUS reporter transgenes.

Fig. S8 Flagellin22 (flg22)-induced expression mediated by the −442 to −321 PROPEP3 promoter in Arabidopsis thaliana seedlings.

Fig. S9 GUS activity staining of 4-wk-old leaves derived from Arabidopsis thaliana lines carrying indicated PROPEP2B:GUS reporter transgenes treated with flagellin22 (flg22) or a droplet infected with Botrytis cinerea spores.

Fig. S10 GUS activity staining of 4-wk-old leaves derived from Arabidopsis thaliana lines carrying indicated PROPEP3C:GUS reporter transgenes treated with flagellin22 (flg22) or a droplet infected with Botrytis cinerea spores.

Fig. S11 Direct microbe-associated molecular pattern (MAMP)-dependent binding of WRKY33 to PROPEP gene promoters.

Fig. S12 Scaffolds containing the DNA sequences of the PROPEP3 promoter and gene, respectively, from Arabis alpina.

nph12233-sup-0002-TableS1.xlsxapplication/msexcel10KTable S1 List of primers used