Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion
Version of Record online: 10 APR 2013
No claim to original French government works New Phytologist © 2013 New Phytologist Trust
Volume 199, Issue 2, pages 505–519, July 2013
How to Cite
Vieira, P., Escudero, C., Rodiuc, N., Boruc, J., Russinova, E., Glab, N., Mota, M., De Veylder, L., Abad, P., Engler, G. and de Almeida Engler, J. (2013), Ectopic expression of Kip-related proteins restrains root-knot nematode-feeding site expansion. New Phytologist, 199: 505–519. doi: 10.1111/nph.12255
- Issue online: 19 JUN 2013
- Version of Record online: 10 APR 2013
- Manuscript Accepted: 1 MAR 2013
- Manuscript Received: 24 JAN 2013
- Fundação para a Ciência e Tecnologia. Grant Number: SFHR\BD\41339\2007
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 Expression patterns in uninfected roots of seven KRPpro:GUS Arabidopsis lines.
Fig. S2 Expression patterns in whole-mount galls of seven KRPpro:GUS Arabidopsis lines.
Fig. S3 Expression patterns of KRP1pro:GUS, KRP3pro:GUS, KRP4pro:GUS and KRP7pro:GUS Arabidopsis lines during gall development.
Fig. S4 In situ localization of KRP1, KRP3, KRP4 and KRP7 mRNAs in Arabidopsis roots infected with M. incognita.
Fig. S5 Characterization of krp loss-of-function lines.
Fig. S6 Mitotic images are often seen in giant cells of the krp2 line.
Fig. S7 Nematode infection tests of krp loss-of-function lines compared with wild type.
Fig. S8 Plant morphology of KRP overexpression lines used for M. incognita inoculation assays.
Fig. S9 Electron micrographs of galls of the KRP2OE line and Arabidopsis wild-type plants.
Fig. S10 Nuclear flow cytometry analyses of uninfected roots in wild type, KRP1OE and KRP2OE lines.
Fig. S11 KRP1-GFP and free-GFP protein expression during gall development.
Table S1 List of primers
Table S2 Summary of expression patterns of seven KRPpro:GUS Arabidopsis lines during gall development
|nph12255-sup-0002-MovieS1a.mov||video/mov||1864K||Movie S1 3D confocal projections of serial optical sections of a 30-d-old gall induced by M. incognita in Arabidopsis wild-type roots.|
|nph12255-sup-0004-MovieS2a.mov||video/mov||1874K||Movie S2 3D confocal projections of serial optical sections of a 40-d-old gall induced by M. incognita in the KRP1OE line.|
|nph12255-sup-0006-MovieS3a.mov||video/mov||1859K||Movie S3 3D confocal projections of serial optical sections of a 40-d-old gall induced by M. incognita in the KRP2OE line.|
|nph12255-sup-0008-MovieS4.mov||video/mov||1950K||Movie S4 GFP-KRP2 expression in tobacco BY2 cells during cell division.|
|nph12255-sup-0009-MovieS5.mov||video/mov||4068K||Movie S5 GFP-KRP2 protein level is regulated by the root-knot nematode during gall development.|