Geminivirus Rep protein interferes with the plant DNA methylation machinery and suppresses transcriptional gene silencing
Article first published online: 24 APR 2013
No claim to original Spanish government works. New Phytologist © 2013 New Phytologist Trust
Volume 199, Issue 2, pages 464–475, July 2013
How to Cite
Rodríguez-Negrete, E., Lozano-Durán, R., Piedra-Aguilera, A., Cruzado, L., Bejarano, E. R. and Castillo, A. G. (2013), Geminivirus Rep protein interferes with the plant DNA methylation machinery and suppresses transcriptional gene silencing. New Phytologist, 199: 464–475. doi: 10.1111/nph.12286
- Issue published online: 19 JUN 2013
- Article first published online: 24 APR 2013
- Manuscript Accepted: 13 MAR 2013
- Manuscript Received: 11 DEC 2012
- Spanish Ministerio de Ciencia y Tecnología. Grant Number: AGL2010-22287-C02-02
- Junta de Andalucía. Grant Number: P07-CVI-02605
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Fig. S1 Protein comparison of DNA methyltranferases and demethylases from different plant species.
Fig. S2 Viral DNA accumulation and Rep expression in N. benthamiana local and systemic infection assays.
Fig. S3 Viral accumulation and Rep expression in a TYLCSV mutant. Transient expression of TYLCSV C2, C4, V2 and Rep in N. benthamiana leaves is shown.
Fig. S4 Transient expression of mutated versions of TGMV and TYLCSV Rep proteins in N. benthamiana.
Fig. S5 Rep, AtDRM2 and AtROS1 expression levels in Arabidopsis thaliana.
Fig. S6 Retrotransposon AtTa3 transcripts were detected in the Arabidopsis thaliana ddm1 mutant.
Fig. S7 TLCSV Rep protein reduces CG methylation in Arabidopsis thaliana.
Fig. S8 Rep expression in Arabidopsis thaliana L5 transgenic plants (RCL1).
Table S1 Primers used in this work
Table S2 Probes, RT-PCR and primers used in this work
Table S3 Plasmids generated in this work
Table S4 Infectious clones used in this work
Methods S1 Supplemental experimental procedures.