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Fig. S1 Comparison of the amino acid sequences of cotton GhPIPs.

Fig. S2 GhPIP gene structures.

Fig. S3 BiFC visualization of GhPIP2s heterodimerization and GhPIP2;6–GhBCP2/4 complex in onion inner epidermal cells.

Fig. S4 Yeast two-hybrid assays of the interactions of GhPIP2s with GhBCPs.

Fig. S5 Quantitative RT-PCR analysis of expression of GhPIP2 genes in GhPIP2;6 RNAi transgenic cotton fibres and ovules.

Table S1 Primers used in gene-specific RT-PCR of GhPIPs

Table S2 Statistical analysis of the length of various fission yeast cell lines