Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development
Article first published online: 9 MAY 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 199, Issue 3, pages 695–707, August 2013
How to Cite
Li, D.-D., Ruan, X.-M., Zhang, J., Wu, Y.-J., Wang, X.-L. and Li, X.-B. (2013), Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development. New Phytologist, 199: 695–707. doi: 10.1111/nph.12309
- Issue published online: 11 JUL 2013
- Article first published online: 9 MAY 2013
- Manuscript Accepted: 3 APR 2013
- Manuscript Received: 20 FEB 2013
- the National Natural Sciences Foundation of China. Grant Numbers: 31000529, 31171174
- Ministry of Agriculture of China. Grant Number: 2011ZX08009-003
Fig. S1 Comparison of the amino acid sequences of cotton GhPIPs.
Fig. S2 GhPIP gene structures.
Fig. S3 BiFC visualization of GhPIP2s heterodimerization and GhPIP2;6–GhBCP2/4 complex in onion inner epidermal cells.
Fig. S4 Yeast two-hybrid assays of the interactions of GhPIP2s with GhBCPs.
Fig. S5 Quantitative RT-PCR analysis of expression of GhPIP2 genes in GhPIP2;6 RNAi transgenic cotton fibres and ovules.
Table S1 Primers used in gene-specific RT-PCR of GhPIPs
Table S2 Statistical analysis of the length of various fission yeast cell lines