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Fig. S1 Silencing of MPK4 does not change salicylic acid (SA) accumulation after elicitations in Nicotiana attenuata.

Fig. S2 irMPK4 plants have normal induced levels of salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) activity after Manduca sexta herbivory.

Fig. S3 Wounding- and herbivory-induced plant green leaf volatiles in wild-type and irMPK4 plants.

Fig. S4 Silencing of COI1 in irMPK4 plants abolishes the accumulation of jasmonic acid (JA)-inducible defensive metabolites.

Fig. S5 Principal component analysis after Pareto scaling of the secondary metabolites detected by ultraperformance liquid chromatography-time of flight-mass spectrometry (UPLC-ToF-MS).

Fig. S6 Total protein and starch contents in wild-type and MPK4-silenced plants.

Fig. S7 The jasmonic acid (JA)-independent defense pathway does not inhibit the growth of Spodoptera littoralis.

Table S1 Ions differentially detected in wild-type (WT), irMPK4, irCOI1 and irMPK4 × irCOI1 plants

Table S2 Ions differentially detected in untreated wild-type (WT) and irMPK4 plants