Nicotiana attenuata MPK4 suppresses a novel jasmonic acid (JA) signaling-independent defense pathway against the specialist insect Manduca sexta, but is not required for the resistance to the generalist Spodoptera littoralis
Version of Record online: 15 MAY 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 199, Issue 3, pages 787–799, August 2013
How to Cite
Hettenhausen, C., Baldwin, I. T. and Wu, J. (2013), Nicotiana attenuata MPK4 suppresses a novel jasmonic acid (JA) signaling-independent defense pathway against the specialist insect Manduca sexta, but is not required for the resistance to the generalist Spodoptera littoralis. New Phytologist, 199: 787–799. doi: 10.1111/nph.12312
- Issue online: 11 JUL 2013
- Version of Record online: 15 MAY 2013
- Manuscript Accepted: 3 APR 2013
- Manuscript Received: 20 DEC 2012
- ClockworkGreen. Grant Number: No. 293926
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Fig. S1 Silencing of MPK4 does not change salicylic acid (SA) accumulation after elicitations in Nicotiana attenuata.
Fig. S2 irMPK4 plants have normal induced levels of salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) activity after Manduca sexta herbivory.
Fig. S3 Wounding- and herbivory-induced plant green leaf volatiles in wild-type and irMPK4 plants.
Fig. S4 Silencing of COI1 in irMPK4 plants abolishes the accumulation of jasmonic acid (JA)-inducible defensive metabolites.
Fig. S5 Principal component analysis after Pareto scaling of the secondary metabolites detected by ultraperformance liquid chromatography-time of flight-mass spectrometry (UPLC-ToF-MS).
Fig. S6 Total protein and starch contents in wild-type and MPK4-silenced plants.
Fig. S7 The jasmonic acid (JA)-independent defense pathway does not inhibit the growth of Spodoptera littoralis.
Table S1 Ions differentially detected in wild-type (WT), irMPK4, irCOI1 and irMPK4 × irCOI1 plants
Table S2 Ions differentially detected in untreated wild-type (WT) and irMPK4 plants