Uncommon ectomycorrhizal networks: richness and distribution of Alnus-associating ectomycorrhizal fungal communities

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(Author for correspondence: tel +1 315 4706794; email trhorton@esf.edu)

The level of specificity is a key evolutionary and ecological parameter of any symbiotic interaction. Where diverse classes of mutualists interact, extracting the drivers of spatial–temporal variation in community composition is also fundamental to understanding the interaction. Ectomycorrhizal (EM) plants and fungi typically associate with many symbionts both as individuals and across a species' range. Trappe (1977) estimated that Pseudotsuga menziesii is compatible with some 2000 species of EM fungi, an impressively high number that has stood the test of time but could be revisited. At the low end among autotrophic plants is the genus Alnus. Although it is known that the EM fungi associated with Alnus are not a rich community (Molina et al., 1992), there is still debate about the factors that might drive this phenomenon, as well as its implications. In this issue of New Phytologist, two contributions on Alnus EM fungi help to fill this gap. Roy et al. (pp. 1228–1238) provide a detailed investigation of the fungal diversity of Alnus associates at a regional scale, while Põlme et al. (pp. 1239–1249) assess fungal biogeography at a global scale. Both studies carefully evaluate the influences of host phylogeny, geographic distance, climate and other environmental variables on patterns of plant–fungus association. We have entered a new era of molecular ecology research in which advanced molecular tools for documentation of microbial diversity and genetic variation are increasingly combined with sophisticated statistical tools of population genetics, phylogenetics and community ecology to provide deeper insights with rigorous scientific support. The simultaneous publication of two studies using the full range of these tools focusing on the same model system at two spatial scales exemplifies this trend, together providing important new insight into host and habitat factors driving host specificity and community dynamics.

Roy et al. sampled 165 trees belonging to five species in France and Corsica. Põlme et al. include root samples of 22 Alnus species collected from 96 stands across the broad geographic range of the genus. Surprisingly, this substantial difference in sampling scale yielded only a modest difference in EM fungal richness. Roy et al. report 86 molecular operational taxonomic units (MOTUs) from 1178 sequences in France and Corsica, while Põlme et al. report 146 MOTUs from 1172 sequences across the globe.

One of the important questions in EM ecology is what drives the composition and species richness of EM fungi found in plant communities (Bruns, 1995). The genus Alnus, with its low richness of EM fungi, provides an excellent model system to address this question. Põlme et al. found host species' phylogenetic relatedness had the greatest impact on EM fungal community composition, suggesting fungus-to-plant specificity at the subgeneric level. The second most important factor driving EM community composition was biogeography. The fungal communities recovered from northwest America shared the most MOTUs with those from Asia, while those from northeast America shared the most MOTUs with those from South America. The biogeographic patterns are not particularly well supported, but are not unique to Alnus fungi: the authors suggest they reflect host migration after the last glacial maximum. Surprisingly, higher richness at the global scale was correlated with increasing soil calcium (Ca) concentration, highlighting the need for additional research focused on mycorrhizal interactions and Ca biogeochemistry.

‘Trappe (1977) estimated that Pseudotsuga menziesii is compatible with some 2000 species of EM fungi, an impressively high number that has stood the test of time but could be revisited.'

Roy et al. found that richness (alpha diversity) was significantly influenced by soil (mineral vs organic) but not host species or subgenus. Unlike Põlme et al., these authors did not detect an influence of Ca concentration on EM fungal diversity, while soil organic matter, carbon (C), nitrogen (N), phosphorus (P) and potassium (K) all influenced fungal community composition across sites (beta diversity). These soil parameters in combination with host explained 26% of the variation of the community structure in the Alnus stands sampled. While altitude may have shaped the communities, the authors could not rule out the role of host specificity at the subgenus level. Host, soil parameters, altitude and longitude all influenced community similarity.

It is worth mentioning that the most frequently encountered fungi showed the lowest specificity in these studies. This pattern is predicted for mutualistic networks, and has been found in numerous symbioses, including mycorrhizas (Bascompte & Jordano, 2007; Martos et al., 2012). Perhaps this is a sampling issue in this case – the chance of not encountering a fungus–host pairing is greatest for uncommon fungi. But it is also interesting to speculate whether the frequency of common fungi is caused by their low specificity: are they frequent because associating with a greater number of hosts facilitates their establishment? Along these lines, Vanderplank (1978) suggested that resistance to a mutualist must reduce fitness, because mutualists (by definition) enhance fitness. But then, why are some fungi narrowly host-specific at the genus or even subgenus level, like Naucoria spp. are with Alnus or Alboalnus?

Perhaps the specificity is related to the nutrient needs and physiology of the symbionts (see Fig. 1). No conclusive link between engaging in an N-fixing symbiosis (as Alnus does with the actinomycete Frankia) and EM specificity has been demonstrated. The coincidence of two unusual ecological features (N-fixation and specificity) invites speculation about a causal link. Much has been written about the evolutionary dynamics resulting in specificity in mutualisms (Thompson, 1994; Hoeksema, 1999). We speculate that Alnus' high P demand, in conjunction with a reduced need for fungus-derived N, provides selection pressure for the plant to associate with fungi that are P specialists but may provide little N (Fig. 1). However, explaining the selective pressure for specialization on Alnus by the fungi is a major theoretical challenge. We must also keep in mind that other nutrients may be involved in these dynamics. For instance, Põlme et al. report strong community responses to Ca. The Ca effect might involve the survival or performance of fungi in soil, or might be mediated indirectly, that is, through Alnus ecophysiology and partner choice. However, if EM fungi protected plants from excess Ca, we would expect a lower richness of EM fungi with increased Ca. It has recently been shown that the same Alnus species associate with different Frankia across a strong local gradient in N and P forms and availability, and it has been suggested that these patterns arise from plant choice rather than direct effects on the bacteria (Anderson et al., 2013). Clearly there is more work to be done on the mechanisms that drive specificity phenomena in mycorrhizal symbioses and we feel the science is primed to investigate the role of physiology.

Figure 1.

Conceptual schematic of ectomycorrhizal (EM) networks. Mycelial networks associated with EM hosts in mixed forests and Alnus are made of many species and individuals of EM fungi. The tree roots and fungi likely explore common zones in the soils. The question mark indicates that we are unclear how the species interact when they explore the same zones. Frankia derived nitrogen (N) is harvested from Alnus litter and root nodules by Alnus and forest EM fungi. However, the fungi apparently do not frequently form common mycorrhizal networks by colonizing both Alnus and forest tree roots. The hypothesized importance of nutrients to the organisms involved is shown by the size of the font. For example, bold, large font for phosphorus (P) and smaller font for N in the Alnus organic N and P window indicates our hypothesis that Alnus-associated fungi are important for the acquisition of P but less so for N and the dynamic is reversed in the forest organic N and P window. The schematic shows other hypotheses about the sources of N and P but it is not meant to be exhaustive. Indeed, we look forward to adding hypotheses about calcium dynamics to this schematic.

While both Roy et al. and Põlme et al. emphasize the importance of host identity in determining fungal community structure and richness, both studies also note significant spatial structure. Põlme et al. report broad-scale biogeographical trends which are largely congruent with historical plant host migrational patterns, although several MOTUs have unexpected intercontinental distributions. Roy et al. report little evidence for large-scale geographic structure in Alnus fungi across France and Corsica and emphasize the role of local-scale phenomena over geography in determining communities. Both studies report that several Tomentella MOTUs have broad ranges, while some other groups such as Geopora were much more limited in extent. As Põlme et al. point out, determining the extent to which limited ranges reflect dispersal abilities versus, for example, edaphic specialization, is difficult at this time. However, the substantial ranges of some MOTUs such as Tomentella sublilacina OTU2, which Põlme et al. report from every region they sampled, strongly suggests that at least some fungi have undergone remarkable migrations. While ITS data alone do not reveal whether there is current gene flow between these disjunct populations, the colonization of several continents itself testifies to impressive dispersal abilities across geologic time, likely since the last glacial maximum (Vincenot et al., 2012). The strong evidence for co-migration of Alnus and its fungi reported in both papers is important, again setting this interaction apart from most EM symbioses in which the linkages are not so tight.

EM fungus communities are impressively diverse, even for a plant genus such as Alnus that is associated with a species-poor group of fungi exhibiting narrow host specificity. Both of these studies report that while the depauperate EM fungal community associated with Alnus allowed better insight into the factors contributing to species distributions, the majority of community variation remained unexplained by the parameters investigated. Further, neither study exhausted the species-effort curve and we do not know which MOTUs may have been at a site but not sampled. We therefore suggest that inferences about the effects of host, edaphic and climatic factors on EM fungal community diversity should be made cautiously. Still, several key lessons emerge from these studies. Both showed (1) a variety of factors correlated with fungal diversity at different spatial scales; (2) fungal species vary in their response to these factors; (3) the fungi associated with Alnus range from those that associate freely with any available Alnus species to those that are specific at the subgenus level; and (4) co-migration has created strong linkages between plant histories and fungal diversity.

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