Breaking the DNA-binding code of Ralstonia solanacearum TAL effectors provides new possibilities to generate plant resistance genes against bacterial wilt disease
Version of Record online: 21 MAY 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 199, Issue 3, pages 773–786, August 2013
How to Cite
de Lange, O., Schreiber, T., Schandry, N., Radeck, J., Braun, K. H., Koszinowski, J., Heuer, H., Strauß, A. and Lahaye, T. (2013), Breaking the DNA-binding code of Ralstonia solanacearum TAL effectors provides new possibilities to generate plant resistance genes against bacterial wilt disease. New Phytologist, 199: 773–786. doi: 10.1111/nph.12324
- Issue online: 11 JUL 2013
- Version of Record online: 21 MAY 2013
- Manuscript Accepted: 3 APR 2013
- Manuscript Received: 5 MAR 2013
- Deutsche Forschungsgemeinschaft. Grant Number: SFB924
- Two Blades Foundation
- Exzellenznetzwerk Biowissenschaften
Vol. 205, Issue 3, 1368, Version of Record online: 3 OCT 2014
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Fig. S1 Annotated sequences of Brg11 and AvrBs3.
Fig. S2 Amino acid sequences of Hpx17 and corresponding truncation derivatives used in subcellular localization studies.
Fig. S3 Alignment of C-terminal regions of AvrBs3 and Brg11 (a) and the sequences of the C-terminal chimeras CTC and CTC-ΔAD (b).
Fig. S4 Alignments of Brg11 and AvrBs3 core repeats.
Fig. S5 Representative amino acid sequences used in the trimer test.
Fig. S6 Reciprocal exchange of RVDs between Brg11 repeats 12 and 8/11 leading to exchange of DNA recognition specificity.
Fig. S7 Nucleotide sequences of brg11 repeats used to assemble three identical, tandem-arranged repeat blocks that were tested in the context of dTALEEBE Brg11.
Fig. S8 Alignment of the N-terminal regions of AvrBs3 and Brg11 (a) and sequence of chimeras NTC1 and NTC2 (b).
Fig. S9 Individual amino acid sequences of RipTALs analyzed in this study.
Fig. S10 Amino acid sequence alignment of RipTALs analyzed in this study.
Fig. S11 Zero base preferences of RipTALI-6, -9, -11 and -14.
Fig. S12 Comparison of predicted Brg11 binding sequences from this study and from Streubel et al. (2012).
Fig. S13 Alignment of Brg11 repeats 2 and 9 and a consensus AvrBs3 repeat.
Table S1 Sequences of primers used in this study
Table S2 Origin of RipTALs analyzed in this study
Table S3 GUS activities determined in the trimer test