MutMap-Gap: whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii
Article first published online: 24 JUN 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 200, Issue 1, pages 276–283, October 2013
How to Cite
Takagi, H., Uemura, A., Yaegashi, H., Tamiru, M., Abe, A., Mitsuoka, C., Utsushi, H., Natsume, S., Kanzaki, H., Matsumura, H., Saitoh, H., Yoshida, K., Cano, L. M., Kamoun, S. and Terauchi, R. (2013), MutMap-Gap: whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii. New Phytologist, 200: 276–283. doi: 10.1111/nph.12369
- Issue published online: 26 AUG 2013
- Article first published online: 24 JUN 2013
- Manuscript Accepted: 13 MAY 2013
- Manuscript Received: 8 MAR 2013
- Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Ministry of Agriculture, Forestry
- Fisheries of Japan
- Ministry of Education, Cultures, Sports and Technology, Japan. Grant Numbers: 23113009, 24248004
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Fig. S1 Flow chart for MutMap-Gap analysis.
Fig. S2 SNP index plots for the 12 rice chromosomes corresponding to bulk sequencing and MutMap analysis of Hit5948 and Hit6780.
Fig. S3 Summary of de novo assembly.
Fig. S4 Confirmation of the splicing error caused by the Hit6780 mutation.
Fig. S5 The association between the Pii phenotype and CAPS marker polymorphism.
Fig. S6 The Pii gene structure.
Table S1 Summary of Illumina GAIIx sequencing and alignment data
Table S2 SNPs with an SNP index of 1 within the Hit5984 candidate region
Table S3 SNPs with an SNP index of 1 within the Hit6780 candidate region