These authors contributed equally to this work.
A genome-wide screen for ethylene-induced Ethylene Response Factors (ERFs) in hybrid aspen stem identifies ERF genes that modify stem growth and wood properties
Article first published online: 1 JUL 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Special Issue: Featured papers on ‘Drought-induced forest mortality’
Volume 200, Issue 2, pages 511–522, October 2013
How to Cite
Vahala, J., Felten, J., Love, J., Gorzsás, A., Gerber, L., Lamminmäki, A., Kangasjärvi, J. and Sundberg, B. (2013), A genome-wide screen for ethylene-induced Ethylene Response Factors (ERFs) in hybrid aspen stem identifies ERF genes that modify stem growth and wood properties. New Phytologist, 200: 511–522. doi: 10.1111/nph.12386
- Issue published online: 18 SEP 2013
- Article first published online: 1 JUL 2013
- Manuscript Accepted: 24 MAY 2013
- Manuscript Received: 29 APR 2013
- US Department of Energy. Grant Number: DE-AC02-05CH11231
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 Alignment of ERF domains of 170 ERF family members identified from Populus trichocarpa genome.
Fig. S3 Response classes of ERF genes after ACC and ethylene treatment.
Fig. S4 Frequency of ERF response by phylogenetic groups.
Fig. S5 ERF transcript abundance in pLMX5::ERF-lines with altered chemotypes.
Fig. S6 Relative difference of diameter and height growth of pLMX5::ERF overexpressing trees to wild type in the second experiment.
Fig. S7 OPLS-DA models of diffuse reflectance FT-IR spectra from wood of pLMX5::ERF trees and spectral band position assignment.
Table S1 Oligonucleotide primer sequences.
Table S3 Populus trichocarpa ERF abbreviations used in this study, corresponding locus names and gene models based on genome assemblies v2.2 and 3.0 at Phytozome.
Table S6 Chemical phenotypes in ERF overexpressors revealed by FT-IR spectroscopy combined with OPLS-DA data analysis in the second experiment.
|nph12386-sup-0002-FigS2.pdf||application/PDF||55K||Fig. S2 Phylogenetic analysis and subgrouping of the 170 predicted Populus trichocarpa ERF proteins based on genome assemblies v2.2 and 3.0 at Phytozome.|
|nph12386-sup-0003-TableS2.xlsx||application/msexcel||15K||Table S2 Details on RNA preparation, cDNA synthesis and qPCR according to MIQE précis|
|nph12386-sup-0004-TableS4.xlsx||application/msexcel||51K||Table S4 Primary expression data of ERF genes in response to ACC and ethylene treatments and during tension wood and normal wood formation|
|nph12386-sup-0005-TableS5.xlsx||application/msexcel||16K||Table S5 qPCR screen of in vitro-grown pLMX5::ERF-lines|