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nph12386-sup-0001-FigS1_S3-S7_TableS1_S3_S6.pdfapplication/PDF776K

Fig. S1 Alignment of ERF domains of 170 ERF family members identified from Populus trichocarpa genome.

Fig. S3 Response classes of ERF genes after ACC and ethylene treatment.

Fig. S4 Frequency of ERF response by phylogenetic groups.

Fig. S5 ERF transcript abundance in pLMX5::ERF-lines with altered chemotypes.

Fig. S6 Relative difference of diameter and height growth of pLMX5::ERF overexpressing trees to wild type in the second experiment.

Fig. S7 OPLS-DA models of diffuse reflectance FT-IR spectra from wood of pLMX5::ERF trees and spectral band position assignment.

Table S1 Oligonucleotide primer sequences.

Table S3 Populus trichocarpa ERF abbreviations used in this study, corresponding locus names and gene models based on genome assemblies v2.2 and 3.0 at Phytozome.

Table S6 Chemical phenotypes in ERF overexpressors revealed by FT-IR spectroscopy combined with OPLS-DA data analysis in the second experiment.

nph12386-sup-0002-FigS2.pdfapplication/PDF55KFig. S2 Phylogenetic analysis and subgrouping of the 170 predicted Populus trichocarpa ERF proteins based on genome assemblies v2.2 and 3.0 at Phytozome.
nph12386-sup-0003-TableS2.xlsxapplication/msexcel15KTable S2 Details on RNA preparation, cDNA synthesis and qPCR according to MIQE précis
nph12386-sup-0004-TableS4.xlsxapplication/msexcel51KTable S4 Primary expression data of ERF genes in response to ACC and ethylene treatments and during tension wood and normal wood formation
nph12386-sup-0005-TableS5.xlsxapplication/msexcel16KTable S5 qPCR screen of in vitro-grown pLMX5::ERF-lines