These authors contributed equally to this work.
Increasing atmospheric CO2 reduces metabolic and physiological differences between isoprene- and non-isoprene-emitting poplars
Article first published online: 4 JUL 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Special Issue: Featured papers on ‘Drought-induced forest mortality’
Volume 200, Issue 2, pages 534–546, October 2013
How to Cite
Way, D. A., Ghirardo, A., Kanawati, B., Esperschütz, J., Monson, R. K., Jackson, R. B., Schmitt-Kopplin, P. and Schnitzler, J.-P. (2013), Increasing atmospheric CO2 reduces metabolic and physiological differences between isoprene- and non-isoprene-emitting poplars. New Phytologist, 200: 534–546. doi: 10.1111/nph.12391
- Issue published online: 18 SEP 2013
- Article first published online: 4 JUL 2013
- Manuscript Accepted: 24 MAY 2013
- Manuscript Received: 7 MAR 2013
- Natural Sciences and Engineering Research Council of Canada
- US Department of Agriculture. Grant Number: #2011-67003-30222
- US Department of Energy. Grant Number: #DE-SC0006967
- Science Foundation. Grant Number: #2010320
- Human Frontier Science Programme (HFSP). Grant Number: #DE-FG02-95ER62083
- CO 2 ;
- Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR-MS);
- nontargeted metabolomics;
- Isoprene, a volatile organic compound produced by some plant species, enhances abiotic stress tolerance under current atmospheric CO2 concentrations, but its biosynthesis is negatively correlated with CO2 concentrations. We hypothesized that losing the capacity to produce isoprene would require stronger up-regulation of other stress tolerance mechanisms at low CO2 than at higher CO2 concentrations.
- We compared metabolite profiles and physiological performance in poplars (Populus × canescens) with either wild-type or RNAi-suppressed isoprene emission capacity grown at pre-industrial low, current atmospheric, and future high CO2 concentrations (190, 390 and 590 ppm CO2, respectively).
- Suppression of isoprene biosynthesis led to significant rearrangement of the leaf metabolome, increasing stress tolerance responses such as xanthophyll cycle pigment de-epoxidation and antioxidant levels, as well as altering lipid, carbon and nitrogen metabolism. Metabolic and physiological differences between isoprene-emitting and suppressed lines diminished as growth CO2 concentrations rose.
- The CO2 dependence of our results indicates that the effects of isoprene biosynthesis are strongest at pre-industrial CO2 concentrations. Rising CO2 may reduce the beneficial effects of biogenic isoprene emission, with implications for species competition. This has potential consequences for future climate warming, as isoprene emitted from vegetation has strong effects on global atmospheric chemistry.