A novel 5-enolpyruvoylshikimate-3-phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide
Article first published online: 1 AUG 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 202, Issue 2, pages 679–688, April 2014
How to Cite
Wang, W., Xia, H., Yang, X., Xu, T., Si, H. J., Cai, X. X., Wang, F., Su, J., Snow, A. A. and Lu, B.-R. (2014), A novel 5-enolpyruvoylshikimate-3-phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide. New Phytologist, 202: 679–688. doi: 10.1111/nph.12428
- Issue published online: 20 MAR 2014
- Article first published online: 1 AUG 2013
- Manuscript Accepted: 27 JUN 2013
- Manuscript Received: 10 APR 2013
- the Natural Science Foundation of China. Grant Numbers: 31271683, and 30730066
- ‘973’ program. Grant Number: 2011CB100401
- the National Program of Development of Transgenic New Species of China. Grant Number: 2013ZX08011-006
- The Ohio State University
- 2002. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Science 50: 721–730. , , , , , , , , .
- 2012. Impacts of genetically engineered crops on pesticide use in the U.S. – the first sixteen years. Environmental Sciences Europe 24: 24. .
- 2010. Field evaluation of reduced-growth, glyphosate-resistant Kentucky bluegrass in a noncompetitive setting. Crop Science 50: 1048–1056. , , .
- 2011. Fate of glyphosate-resistant giant ragweed (Ambrosia trifida) in the presence and absence of glyphosate. Weed Science 59: 506–511. , , .
- 2004. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Annals of Botany 93: 67–73. , , , , .
- 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744. , , , , , .
- 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science 22: 370–371. , , .
- 1966. A proposal for a uniform nomenclature in bacterial genetics. Genetics 54: 61–76. , , , .
- 2006. Physiological and molecular insight on the mechanisms of resistance to glyphosate in Conyza canadensis (L.) Cronq. biotypes. Pesticide Biochemistry and Physiology 86: 30–41. , , , , , .
- 2008. Physiological and molecular bases of glyphosate resistance in Conyza canadensis biotypes from Spain. Weed Research 48: 257–265. , , , , , .
- European Food Safety Authority (EFSA). 2007. Opinion of the Scientific Panel on Genetically Modified Organisms on applications (references EFSA-GMO-UK-2005-19 and EFSA-GMO-RX-GA21) for the placing on the market of glyphosate-tolerant genetically modified maize GA21. EFSA Journal 541: 1–25.
- 1997. Glyphosate: a unique global herbicide. Washington, DC, USA: American Chemistry Society. , , .
- 2006. Molecular basis for the herbicide resistance of Roundup Ready crops. Proceedings of the National Academy of Sciences, USA 103: 13010–13015. , , , , .
- 2010. Gene amplification confers glyphosate resistance in Amaranthus palmeri. Proceedings of the National Academy of Sciences, USA 107: 1029–1034. , , , , , , , , , et al.
- 2009. Evolution of glyphosate-resistant crop technology. Weed Science 57: 108–117. .
- 2012. Tracking the Roundup Ready® gene: implications for coexistence. Proceedings for the 2012 Winter Seed School, Western Alfalfa Seed Growers Conference, January 15–17, Las Vegas, NV, USA, 53–55. .
- 1999. Tandem constructs: preventing the rise of superweeds. Trends in Biotechnology 17: 361–366. .
- 2009. A strategy to provide long-term control of weedy rice while mitigating herbicide resistance transgene flow, and its potential use for other crops with related weeds. Pest Management Science 65: 723–731. , .
- 2009. International survey of herbicide resistant weeds. [WWW document] URL http://www.weedscience.org [accessed on 15 June 2013]. .
- 1995. The shikimate pathway: early steps in the biosynthesis of aromatic compounds. Plant Cell 7: 907–919. .
- 2012. Global status of commercialized biotech/GM crops. ISAAA Briefs No. 44. Ithaca, NY, USA: ISAAA. .
- 2007. Identification of a new gene coding for EPSPS with high glyphosate resistance from the metagenomic library. Current Microbiology 55: 350–355. , , , , .
- 1992. History of herbicide-tolerant crops, methods of development and current state of the art – emphasis on glyphosate tolerance. Weed Technology 6: 626–634. , , .
- 1987. Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvulshikimate-3-phosphate synthase sequence analysis and manipulation to obtain glyphosate-tolerant plants. Molecular and General Genetics 210: 437–442. , , .
- 2012. A built-in mechanism to mitigate the spread of insect-resistance and herbicide-tolerance in weedy rice populations. PLoS ONE 7: e31625. , , , , , .
- 2005. Gene flow from genetically modified rice and its environmental consequences. BioScience 559: 669–678. , .
- 2009. Gene flow from genetically modified rice to its wild relatives: assessing potential ecological consequences. Biotechnology Advances 27: 1083–1091. , .
- Monsanto. 2002. Safety Assessment of Roundup Ready Corn Event GA21. [WWW document] URL http://www.monsanto.com/products/Documents/safety-summaries/corn_pss_GA21.pdf [accessed on 15 June 2013].
- 1998. Indole-3-acetic acid is synthesized from l-tryptophan in roots of Arabidopsis thaliana. Planta 206: 362–369. , , .
- 2006. Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technology 20: 282–289. , .
- 1997. Herbicide resistance: impact and management. Advances in Agronomy 58: 57–93. , , , .
- 2006. Evolutionary origins of the eukaryotic shikimate pathway: gene fusions, horizontal gene transfer, and endosymbiotic replacements. Eukaryotic Cell 5: 1517–1531. , , , , , , .
- 2012. EPSPS gene amplification in glyphosate-resistant Italian ryegrass (Lolium perenne ssp. multiflorum) from Arkansas. Pest Management Science 68: 1223–1230. , , , , , , .
- 2011. The establishment of genetically engineered canola populations in the US. PLoS ONE 6: e25736. , , , , , , , .
- 1986. Engineering herbicide tolerance in plants. Science 233: 478–481. , , , , , , , , , et al.
- 2011. What have mechanisms of resistance to glyphosate taught us? Pest Management Science 68: 3–9. , , .
- 2007. Gene flow between Clearfield™ rice and red rice. Crop Protection 26: 349–356. , , , , , .
- 2010. Growth, phenology, and intraspecific competition between glyphosate-resistant and glyphosate-susceptible horseweeds (Conyza canadensis) in the San Joaqin Valley of California. Weed Science 58: 147–153. , , , .
- 1986. Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase in a glyphosate-tolerant Petunia hybrida cell line. Archives of Biochemistry and Biophysics 244: 169–178. , , , , .
- 2005. Novel aroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China. Applied and Environmental Microbiology 71: 4771–4776. , , , , , , , , , et al.
- 2011. Improvement of glyphosate resistance through concurrent mutations in three amino acids of the Ochrobactrum 5-enopyuvylshikimate-3-phosphate synthase. Applied and Environmental Microbiology 77: 8409–8414. , , , , , , .
- USDA. 2011a. Scotts Miracle-Gro Co.; regulatory status of Kentucky Bluegrass genetically engineered for herbicide tolerance. Docket No. APHIS-2011-0080. Federal Register 39812, Vol. 76, No. 130, July 7, 2011.
- USDA. 2011b. International Center for Technology Assessment and the Center for Food Safety; noxious weed status of Kentucky Bluegrass genetically engineered for herbicide tolerance. Docket No. APHIS-2011-0081. Federal Register 39811 Vol. 76, No. 130, July 7, 2011.
- 2009. Fitness costs associated with evolved resistance alleles in plants. New Phytologist 184: 751–767. , , .
- 2010. A herbicide-resistant ACCase 1781 Setaria mutant shows higher fitness than wild type. Heredity 105: 394–400. , , , .
- 2008. Do escaped transgenes persist in nature? The case of an herbicide resistance transgene in a weedy Brassica rapa population. Molecular Ecology 17: 1387–1395. , , , .
- 2004. Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proceedings of the National Academy of Sciences, USA 101: 14533–14538. , , , , , , , , .
- 2008. Characterization of selected common lambsquarters (Chenopodium album) biotypes with tolerance to glyphosate. Weed Science 56: 685–691. , , , , , .
- 2002. Cloning of genomic DNA of rice 5-enolpyruvylshikimate 3-phosphate synthase gene and chromosomal localization of the gene. Science in China 45: 251–259. , , , , .
- 2011. Novel AroA from Pseudomonas putida confers tobacco plant with high tolerance to glyphosate. PLoS ONE 6: e19732. , , , , , , , .
- 2011. Transgenes for insect resistance reduce herbivory and enhance fecundity in advanced generations of crop–weed hybrids of rice. Evolutionary Applications 4: 672–684. , , , , , , .
- 2008. Escape and establishment of transgenic glyphosate-resistant creeping bentgrass Agrostis stolonifera in Oregon, USA: a 4-year study. Journal of Applied Ecology 45: 486–494. , , , .
- 2011. Innate factors causing differences in gene flow frequency from transgenic rice to different weedy rice biotypes. Pest Management Science 67: 677–690. , , , , .