Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems
Article first published online: 5 AUG 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 200, Issue 4, pages 1022–1033, December 2013
How to Cite
Fuss, J., Liegmann, O., Krause, K. and Rensing, S. A. (2013), Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems. New Phytologist, 200: 1022–1033. doi: 10.1111/nph.12433
- Issue published online: 4 NOV 2013
- Article first published online: 5 AUG 2013
- Manuscript Accepted: 1 JUL 2013
- Manuscript Received: 5 MAR 2013
- NFR (Norway). Grant Number: 180662/V40
- DAAD (Germany)
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|nph12433-sup-0001-FigS1.docx||Word document||245K||Fig. S1 Performance of GTP_Pp on different test data sets.|
|nph12433-sup-0002-TableS1.xlsx||application/msexcel||75K||Table S1 Training and test data set for ATP2; area under ROC curve|
|nph12433-sup-0003-TableS2.xls||application/msexcel||63K||Table S2 Training and test data set sorted according to method of origin for GTP_Pp|
|nph12433-sup-0004-TableS3.xls||application/msexcel||43K||Table S3 List of all tested features; Top-15 N- and C- terminal feature list used by the two GTP versions|
|nph12433-sup-0005-TableS4.xls||application/msexcel||27K||Table S4 Sequences of primers used for cloning|
|nph12433-sup-0006-TableS5.xls||application/msexcel||29K||Table S5 Comparison of the performance of three prediction tools used for creating the mitochondrial data set with a reduced mitochondrial protein test data set from P. patens|
|nph12433-sup-0007-TableS6.docx||Word document||21K||Table S6 Summary of localization results on the in vivo test set|
Methods S1 Details on the two prediction tools, ATP2 and GTP.
Notes S1 Details on the two prediction tools, ATP2 and GTP.