Nucleocapsid of Tomato spotted wilt tospovirus forms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K
Article first published online: 23 AUG 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 200, Issue 4, pages 1212–1224, December 2013
How to Cite
Feng, Z., Chen, X., Bao, Y., Dong, J., Zhang, Z. and Tao, X. (2013), Nucleocapsid of Tomato spotted wilt tospovirus forms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K. New Phytologist, 200: 1212–1224. doi: 10.1111/nph.12447
- Issue published online: 4 NOV 2013
- Article first published online: 23 AUG 2013
- Manuscript Accepted: 11 JUL 2013
- Manuscript Received: 6 MAY 2013
- National Natural Science Foundation of China. Grant Numbers: 31222045, 31171813
- New Century Excellent Talents in University. Grant Number: NCET-12-0888
- Fok Ying Tung Education Foundation. Grant Number: 131027
- National Program on Key Basic Research Project of China. Grant Number: 2010CB134501
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Fig. S1 Tomato spotted wilt tospovirus (TSWV) N-YFP inclusions co-localize with the endoplasmic reticulum (ER).
Fig. S2 Tomato spotted wilt tospovirus (TSWV) N-YFP inclusions co-localize with the actin filaments.
Fig. S3 Subcellular distribution analysis of YFP-N and N-YFP fusion proteins expressed by agroinfiltration compared with that of native N protein expressed during Tomato spotted wilt tospovirus (TSWV) infection.
Fig. S4 Tomato spotted wilt tospovirus (TSWV) N inclusions associate with microtubules.
Fig. S5 Analysis of virus accumulation in isolated necrotic lesions and the area outside of lesions in latrunculin B (LatB)-treated leaves of Nicotiana tabacum cv Samsun NN.
|nph12447-sup-0002-TableS1.doc||Word document||137K||Table S1 The number and percentage of total lesions in different size ranges in dimethylsulfoxide (DMSO)- and latrunculin B (LatB)-treated leaf halves on Nicotiana tabacum cv Samsun NN after inoculation with Tomato spotted wilt tospovirus (TSWV)|
|nph12447-sup-0004-VideoS1.mov||video/mov||3012K||Video S1 Highly motile cytoplasmic inclusion bodies formed by YFP-N in Nicotiana benthamiana cells.|
|nph12447-sup-0005-VideoS2.mov||video/mov||1708K||Video S2 Highly motile cytoplasmic inclusion bodies formed by N-YFP in Nicotiana benthamiana cells.|
|nph12447-sup-0006-VideoS3.mov||video/mov||3757K||Video S3 Tomato spotted wilt tospovirus (TSWV) YFP-N inclusions trafficking along the cortical endoplasmic reticulum in Nicotiana benthamiana cells.|
|nph12447-sup-0007-VideoS4.mov||video/mov||2007K||Video S4 Tomato spotted wilt tospovirus (TSWV) YFP-N inclusions trafficking along actin filaments in Nicotiana benthamiana cells.|
|nph12447-sup-0008-VideoS5.mov||video/mov||3584K||Video S5 Co-expression of YFP-N, endoplasmic reticulum (ER) (GFP-HDEL) and Golgi stacks (Man49-mCherry) markers in Nicotiana benthamiana cells.|
|nph12447-sup-0009-VideoS6.mov||video/mov||2604K||Video S6 Effects of microtubule inhibitor oryzalin on the intracellular movement of Tomato spotted wilt tospovirus (TSWV) YFP-N-labeled inclusions in Nicotiana benthamiana cells.|