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nph12447-sup-0001-FigS1-S5.docWord document2700K

Fig. S1 Tomato spotted wilt tospovirus (TSWV) N-YFP inclusions co-localize with the endoplasmic reticulum (ER).

Fig. S2 Tomato spotted wilt tospovirus (TSWV) N-YFP inclusions co-localize with the actin filaments.

Fig. S3 Subcellular distribution analysis of YFP-N and N-YFP fusion proteins expressed by agroinfiltration compared with that of native N protein expressed during Tomato spotted wilt tospovirus (TSWV) infection.

Fig. S4 Tomato spotted wilt tospovirus (TSWV) N inclusions associate with microtubules.

Fig. S5 Analysis of virus accumulation in isolated necrotic lesions and the area outside of lesions in latrunculin B (LatB)-treated leaves of Nicotiana tabacum cv Samsun NN.

nph12447-sup-0002-TableS1.docWord document137KTable S1 The number and percentage of total lesions in different size ranges in dimethylsulfoxide (DMSO)- and latrunculin B (LatB)-treated leaf halves on Nicotiana tabacum cv Samsun NN after inoculation with Tomato spotted wilt tospovirus (TSWV)
nph12447-sup-0004-VideoS1.movvideo/mov3012KVideo S1 Highly motile cytoplasmic inclusion bodies formed by YFP-N in Nicotiana benthamiana cells.
nph12447-sup-0005-VideoS2.movvideo/mov1708KVideo S2 Highly motile cytoplasmic inclusion bodies formed by N-YFP in Nicotiana benthamiana cells.
nph12447-sup-0006-VideoS3.movvideo/mov3757KVideo S3 Tomato spotted wilt tospovirus (TSWV) YFP-N inclusions trafficking along the cortical endoplasmic reticulum in Nicotiana benthamiana cells.
nph12447-sup-0007-VideoS4.movvideo/mov2007KVideo S4 Tomato spotted wilt tospovirus (TSWV) YFP-N inclusions trafficking along actin filaments in Nicotiana benthamiana cells.
nph12447-sup-0008-VideoS5.movvideo/mov3584KVideo S5 Co-expression of YFP-N, endoplasmic reticulum (ER) (GFP-HDEL) and Golgi stacks (Man49-mCherry) markers in Nicotiana benthamiana cells.
nph12447-sup-0009-VideoS6.movvideo/mov2604KVideo S6 Effects of microtubule inhibitor oryzalin on the intracellular movement of Tomato spotted wilt tospovirus (TSWV) YFP-N-labeled inclusions in Nicotiana benthamiana cells.
nph12447-sup-0003-legendsVideosS1-S6.docWord document29K