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Fig. S1 Phylogenetic tree of ZIP proteins in Arabidopsis, rice, Brachypodium, barley and wheat.

Fig. S2 Southern blot analysis and semiquantitative RT-PCR analysis of transcript abundances of the HvZIP7 transgene.

Fig. S3 Effects of Zn supplement rates on Fe, Mn and Cu concentrations of shoots and roots in soil-grown plants.

Fig. S4 Effects of Zn concentrations in nutrient solution on dry weight of the plants overexpressing HvZIP7.

Fig. S5 Effects of a luxury range of Zn concentrations in nutrient solution on Fe, Mn and Cu concentrations of shoots and roots.

Fig. S6 Total grain number and 1000 grain weight of plants grown in a potting mix.

Fig. S7 Fe, Mn and Cu concentration and content of barley grains.

Table S1 Identifiers and annotations for the Arabidopsis and rice genes described in this study