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nph12469-sup-0001-FigS1-S11_TabS1.pptapplication/mspowerpoint2656K

Fig. S1 Identification of C-terminal FLAG peptide-tagged transgenic OST1 overexpressing Arabidopsis thaliana lines.

Fig. S2 ABA inhibition of light induced stomatal opening and ABA promotion of stomatal closure in Arabidopsis thaliana wild-type (Ler) and empty vector lines (EV1 and EV3).

Fig. S3 Time courses of stomatal aperture changes induced by 10 μM ABA in Arabidopsis thaliana wild-type (Ler), two empty vector control lines (EV1 and EV3), two OST1 overexpressing lines (OV1 and OV4) and two ost1 mutants (ost1-1 and ost1-2).

Fig. S4 ABA inhibition of whole cell K+in currents in guard cells of Arabidopsis thaliana wild-type (Ler) and empty vector lines (EV1 and EV3).

Fig. S5 Arabidopsis thaliana KAT1 does not interact with AtrbohD.

Fig. S6 ABA activation of whole cell anion currents in guard cells of Arabidopsis thaliana wild-type (Ler) and empty vector lines (EV1 and EV3).

Fig. S7 ABA activation of guard cell anion currents is impaired in Arabidopsis thaliana ost1 mutants under weak pH buffered pipette solution condition.

Fig. S8 Arabidopsis thaliana SLAC1 does not interact with AtrbohF.

Fig. S9 ABA activation of whole cell Ba2+ currents through Ca2+-permeable channels in guard cells of Arabidopsis thaliana wild-type (Ler) and empty vector lines (EV1 and EV3).

Fig. S10 ABA-induced ROS production in guard cells of Arabidopsis thaliana wild-type (Ler) and empty vector lines (EV1 and EV3).

Fig. S11 Arabidopsis thaliana KAT1 does not interact with AtrbohF.

Table S1 Interaction analyses between Arabidopsis thaliana OST1 and K+ channel proteins, KAT1 and KAT2, in yeast (mbSUS assay)

nph12469-sup-0002-VideoS1.avivideo/avi363KVideo S1 ROS imaging in a guard cell pair of Arabidopsis thaliana wild-type (Ler) in response to 50 μM ABA.
nph12469-sup-0003-VideoS2.avivideo/avi542KVideo S2 ROS imaging in a guard cell pair of Arabidopsis thaliana ost1-1 mutant in response to 50 μM ABA.
nph12469-sup-0004-VideoS3.avivideo/avi394KVideo S3 ROS imaging in a guard cell pair of Arabidopsis thaliana ost1-2 mutant in response to 50 μM ABA.
nph12469-sup-0005-VideoS4.avivideo/avi462KVideo S4 ROS imaging in a guard cell pair of Arabidopsis thaliana empty vector line EV1 in response to 50 μM ABA.
nph12469-sup-0006-VideoS5.avivideo/avi356KVideo S5 ROS imaging in a guard cell pair of Arabidopsis thaliana empty vector line EV3 in response to 50 μM ABA.
nph12469-sup-0007-VideoS6.avivideo/avi404KVideo S6 ROS imaging in a guard cell pair of Arabidopsis thaliana OST1 overexpressing line OV1 in response to 50 μM ABA.
nph12469-sup-0008-VideoS7.avivideo/avi395KVideo S7 ROS imaging in a guard cell pair of Arabidopsis thaliana OST1 overexpressing line OV4 in response to 50 μM ABA.
nph12469-sup-0009-VideoS8.avivideo/avi455KVideo S8 ROS imaging in a guard cell pair of Arabidopsis thaliana wild-type (Ler) in response to ethanol.
nph12469-sup-0010-VideoS9.avivideo/avi580KVideo S9 ROS imaging in a guard cell pair of Arabidopsis thaliana ost1-1 mutant in response to ethanol.
nph12469-sup-0011-VideoS10.avivideo/avi386KVideo S10 ROS imaging in a guard cell pair of Arabidopsis thaliana empty vector line EV1 in response to ethanol.
nph12469-sup-0012-VideoS11.avivideo/avi434KVideo S11 ROS imaging in a guard cell pair of Arabidopsis thaliana OST1 overexpressing line OV1 in response to ethanol.