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Fig. S1 Removal of scopoletin and derivatives by Sep-Pak C18 column from the Arabidopsis nutrient solution.

Fig. S2 Analysis of phenolic compound standards by HPLC/ESI-MS(TOF).

Fig. S3 MS2 spectra of coumarin standards and MS3 spectra of compounds 1, 4 and 5. Common losses from the precursors are marked in each spectrum.

Fig. S4 Scopoletin and derivatives eluted from the C18 resin column.

Table S1 Primers used for semi-quantitative RT-PCR

Table S2 List of eluents and standards used

Table S3 Operating conditions of HPLC/ESI-MS(TOF) and HPLC/ESI-MS/MS(ion trap)

Table S4 Manganese, Cu and Zn concentrations (μg g−1 DW) in young leaves and roots from Arabidopsis thaliana plants

Notes S1 Detailed information on the identification of the Arabidopsis fluorescent compounds as scopoletin and derivatives.