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Fig. S1 Motif analysis of OsFIE2 and alignment of OsFIE2 with other major WD40-containing Polycomb group (PcG) homologs in plants and animals.

Fig. S2 Phylogenetic trees of OsFIE2 and other embryonic stem cell (ESC) homologs of plant Polycomb group (PcG) proteins.

Fig. S3 Subcellular localization of OsFIE2 in onion epidermal cells.

Fig. S4 Transformation constructs.

Fig. S5 The statistical result on the pollen sterile ratio shows a high proportion of sterile pollen in the RNAi line.

Fig. S6 Relative expression level of OsFIE1 and OsFIE2 in rice ovary 3 d after pollination (DAP).

Fig. S7 The corresponding phenotypes in primary roots agreed with the relative expression level of OsFIE2 in transgenic lines.

Fig. S8 The expression pattern of OsFIE2 in rice.

Table S1 Statistical and agronomic characteristics of the transformation

Table S2 The statistical characteristics of the RNAi line compared with the wild-type

Table S3 Primers for reverse transcription-polymerase chain reaction (RT-PCR)