The pepper cysteine/histidine-rich DC1 domain protein CaDC1 binds both RNA and DNA and is required for plant cell death and defense response
Article first published online: 7 OCT 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 2, pages 518–530, January 2014
How to Cite
Hwang, I. S., Choi, D. S., Kim, N. H., Kim, D. S. and Hwang, B. K. (2014), The pepper cysteine/histidine-rich DC1 domain protein CaDC1 binds both RNA and DNA and is required for plant cell death and defense response. New Phytologist, 201: 518–530. doi: 10.1111/nph.12521
- Issue published online: 18 DEC 2013
- Article first published online: 7 OCT 2013
- Manuscript Accepted: 20 AUG 2013
- Manuscript Received: 10 JUL 2013
- Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center), Rural Development Administration. Grant Number: PJ008027
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Fig. S1 Nucleotide and deduced amino acid sequences of pepper CaDC1 cDNA.
Fig. S2 Alignment of pepper (Capsicum annuum) CaDC1 to various DC1 proteins.
Fig. S3 Subcellular localization of CaDC1-GFP and the CaDC1-NES-, nes-, NLS- or nls-GFP fusion constructs transiently expressed in Nicotiana benthamiana leaves.
Fig. S4 Induction of the cell death response by CaDC1 transient expression in pepper leaves.
Fig. S5 Effect of subcellular localization of CaDC1 and DC1 domain on induction of the cell death response in pepper leaves.
Fig. S6 Effect of transient expression of 35S:CaDC1, 35S:CaDC1-NLS and 35S:CaDC1C constructs on induction of the cell death response in pepper leaves.
Fig. S7 Enhanced susceptibility of CaDC1-OX Arabidopsis transgenic lines to Pseudomonas syringae pv tomato DC3000 and DC3000 (avrRpm1).
Table S1 Oligonucleotide sequences used for quantitative RT-PCR in this study