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nph12530-sup-0001-FigsS1-S2_MethodS1.docxWord document1733K

Fig. S1 Functional isolation of septal compartments in developing spores in Magnaporthe oryzae measured using fluorescent recovery after photobleaching (FRAP) of Grx1-roGFP2.

Fig. S2 Occlusion of septal pores in developing spores in Magnaporthe oryzae observed using transmission electron microscopy.

Methods S1 Transmission electron microscopy of conidia from Magnaporthe oryzae.

nph12530-sup-0002-VideoS1.wmvvideo/wmv1036KVideo S1 Measurement of the redox potential of the glutathione pool in Magnaporthe oryzae germlings using recombinant Grx1-roGFP2.
nph12530-sup-0003-VideoS2.wmvvideo/wmv396KVideo S2 Measurement of reactive oxygen species (ROS) using 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) in developing spores and during appressorium formation in Magnaporthe oryzae.
nph12530-sup-0004-VideoS3.wmvvideo/wmv1513KVideo S3 Simultaneous measurement of mitochondrial membrane potential, reactive oxygen species (ROS) and Grx1-roGFP2 ratio in a swelling germ tube of Magnaporthe oryzae.
nph12530-sup-0005-VideoS4.wmvvideo/wmv1763KVideo S4 Simultaneous measurement of mitochondrial membrane potential, reactive oxygen species (ROS) and Grx1-roGFP2 ratio in a developing appressorium of Magnaporthe oryzae.
nph12530-sup-0006-VideoS5.wmvvideo/wmv1317KVideo S5 Formation of glutathione-bimane and subsequent vacuolar sequestration in a developing germling of Magnaporthe oryzae.
nph12530-sup-0007-VideoS6.wmvvideo/wmv1122KVideo S6 Animated three-dimensional projections of vacuolar segmentation in spores of Magnaporthe oryzae during the early stages of germination.
nph12530-sup-0008-VideoS7.wmvvideo/wmv1286KVideo S7 Animated three-dimensional projections of Grx1-roGFP2 fluorescence expressed in Magnaporthe oryzae, and the corresponding ratio images, 16 or 24 h after inoculation on a susceptible rice variety, CO39.
nph12530-sup-0009-VideoS8.wmvvideo/wmv9489KVideo S8 Animated three-dimensional projections of Grx1-roGFP2 fluorescence expressed in Magnaporthe oryzae, and the corresponding ratio images, 48 h after inoculation on a susceptible rice variety, CO39.
nph12530-sup-0010-VideoS9.wmvvideo/wmv1427KVideo S9 Animated three-dimensional projections of Grx1-roGFP2 fluorescence expressed in Magnaporthe oryzae, and the corresponding ratio images, 16 or 24 h after inoculation on a resistant rice variety, IR68.
nph12530-sup-0011-VideoS10.wmvvideo/wmv7403KVideo S10 Animated three-dimensional projections of Grx1-roGFP2 fluorescence expressed in Magnaporthe oryzae, and the corresponding ratio images, 48 h after inoculation on a resistant rice variety, IR68.