These authors contributed equally to the work.
AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts
Article first published online: 4 OCT 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 2, pages 452–465, January 2014
How to Cite
Manara, A., DalCorso, G., Leister, D., Jahns, P., Baldan, B. and Furini, A. (2014), AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts. New Phytologist, 201: 452–465. doi: 10.1111/nph.12533
- Issue published online: 18 DEC 2013
- Article first published online: 4 OCT 2013
- Manuscript Accepted: 27 AUG 2013
- Manuscript Received: 26 JUL 2013
- MIUR Italian Ministry of University and Research
- Abc1 protein;
- oxidative stress;
- The Abc1 protein kinases are a large family of functionally diverse proteins with multiple roles in the regulation of respiration and oxidative stress tolerance.
- A functional characterization was carried out for AtSIA1, an Arabidopsis thaliana Abc1-like protein, focusing on its potential redundancy with its homolog AtOSA1. Both proteins are located within chloroplasts, even if a different subplastidial localization seems probable. The comparison of atsia1 and atosa1 mutants, atsia1/atosa1 double mutant and wild-type plants revealed a reduction in plastidial iron-containing proteins of the Cytb6f complex in the mutants. Iron uptake from soil is not hampered in mutant lines, suggesting that AtSIA1 and AtOSA1 affect iron distribution within the chloroplast.
- Mutants accumulated more ferritin and superoxide, and showed reduced tolerance to reactive oxygen species (ROS), potentially indicating a basal role in oxidative stress. The mutants produced higher concentrations of plastochromanol and plastoquinones than wild-type plants, but only atsia1 plants developed larger plastoglobules and contained higher concentrations of α- and γ-tocopherol and VTE1.
- Taken together, these data suggest that AtSIA1 and AtOSA1 probably act in signaling pathways that influence responses to ROS production and oxidative stress.