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nph12533-sup-0001-FigsS1-S9.docWord document4747K

Fig. S1 Functional complementation test of the yeast abc1 mutant.

Fig. S2 AtSIA1 and AtOSA1 gene expression analysis in A. thaliana WT plants.

Fig. S3 Overexpression of chimera AtSIA1-FLAG and AtOSA1-FLAG proteins in transgenic lines (OX-AtSIA1 and OX-AtOSA1).

Fig. S4 Analysis of AtSIA and AtOSA1 localization in chloroplast membranes.

Fig. S5 Blue-Native page of thylakoidal complexes.

Fig. S6 Analysis of heme content in wild-type and mutant plants.

Fig. S7 Expression of iron-related proteins in A. thaliana plants.

Fig. S8 Expression of antioxidant enzyme in iron-deficiency conditions.

Fig. S9 Localization of proteins recognized by antibodies utilized.

nph12533-sup-0002-MethodS1-TableS1.docWord document82K

Table S1 Pigment content determined by HPLC

Methods S1 Details of methods of generation of antibodies against the AtSIA1 and AtOSA1 proteins; yeast complementation assay; total protein extraction and quantification; Blue-Native PAGE; and heme extraction and quantification.