These authors contributed equally to the work.
AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts
Article first published online: 4 OCT 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 2, pages 452–465, January 2014
How to Cite
Manara, A., DalCorso, G., Leister, D., Jahns, P., Baldan, B. and Furini, A. (2014), AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts. New Phytologist, 201: 452–465. doi: 10.1111/nph.12533
- Issue published online: 18 DEC 2013
- Article first published online: 4 OCT 2013
- Manuscript Accepted: 27 AUG 2013
- Manuscript Received: 26 JUL 2013
- MIUR Italian Ministry of University and Research
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Fig. S1 Functional complementation test of the yeast abc1 mutant.
Fig. S2 AtSIA1 and AtOSA1 gene expression analysis in A. thaliana WT plants.
Fig. S3 Overexpression of chimera AtSIA1-FLAG and AtOSA1-FLAG proteins in transgenic lines (OX-AtSIA1 and OX-AtOSA1).
Fig. S4 Analysis of AtSIA and AtOSA1 localization in chloroplast membranes.
Fig. S5 Blue-Native page of thylakoidal complexes.
Fig. S6 Analysis of heme content in wild-type and mutant plants.
Fig. S7 Expression of iron-related proteins in A. thaliana plants.
Fig. S8 Expression of antioxidant enzyme in iron-deficiency conditions.
Fig. S9 Localization of proteins recognized by antibodies utilized.
Table S1 Pigment content determined by HPLC
Methods S1 Details of methods of generation of antibodies against the AtSIA1 and AtOSA1 proteins; yeast complementation assay; total protein extraction and quantification; Blue-Native PAGE; and heme extraction and quantification.