These authors contributed equally to this work.
Towards the discovery of novel genetic component involved in stress resistance in Arabidopsis thaliana
Version of Record online: 18 OCT 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 3, pages 810–824, February 2014
How to Cite
Juraniec, M., Lequeux, H., Hermans, C., Willems, G., Nordborg, M., Schneeberger, K., Salis, P., Vromant, M., Lutts, S. and Verbruggen, N. (2014), Towards the discovery of novel genetic component involved in stress resistance in Arabidopsis thaliana. New Phytologist, 201: 810–824. doi: 10.1111/nph.12554
- Issue online: 9 JAN 2014
- Version of Record online: 18 OCT 2013
- Manuscript Accepted: 16 SEP 2013
- Manuscript Received: 8 AUG 2013
- Belgian National Fund for Scientific Research. Grant Number: FNRS-FRFC 2.4.527.10.F
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Fig. S1 Illustration of the fine-mapping evolution of the cmr1 Arabidopsis mutant.
Fig. S2 Root length of 15-d-old pme3 (At3g14310) seedlings exposed to high-Cu or salt conditions.
Fig. S3 Detection of a mutation at the At3g14190 locus.
Fig. S4 Coverage of cmr1-1 reads onto the Col-0 WT Arabidopsis reference genome.
Fig. S5 Magnification of two low-coverage regions and analysis of cmr1 pair reads.
Fig. S6 Nature of the cmr1 mutation and genomic sequence of the hybrid At3g14190 gene.
Fig. S7 Impact of Cu2+ and Na+ excess on cmr1-1 and WT Arabidopsis biomass in hydroponics.
Fig. S8 Allelism test between Arabidopsis cmr1-1 and T-DNA mutants at the At3g14190 locus.
Table S1 Number of M2 Arabidopsis seedlings screened in vertical plates
Table S2 List of the 53 candidate genes located in the 184 kb region of interest delimited by the fine-mapping of the cmr1 Arabidopsis mutant
Table S3 Microarray analysis of the genes lying in the 184 kb region delimited by the fine-mapping of cmr1 and whose expression was differentially regulated in cmr1 as compared with WT
Table S4 List of T-DNA insertion mutants available at the At3g14190 locus
Table S5 Mineral profile of the in vitro-grown cmr1-2 mutant and WT Arabidopsis plants
Table S6 Potassium concentrations in the in vitro-grown Arabidopsis cmr1-1 mutant and T-DNA SALK_035661 lines
Table S7 Molecular markers used in the map-based cloning of the cmr1 Arabidopsis mutant
Table S8 List of potential cis-acting regulatory elements identified in the 5′ regulatory sequence of the CMR1 Arabidopsis gene
Table S9 List of genes related to the cell cycle differentially regulated in cmr1-1 compared with WT
Table S10 List of genes related to potassium homeostasis differentially regulated in cmr1-1 compared with WT (cutoff = 3)
Table S11 List of primers used in genotyping of both cmr1 mutant alleles (positions of primers are relative to the translation initiation codon) and cloning of CMR1 CDS