DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis
Article first published online: 1 NOV 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 201, Issue 3, pages 825–836, February 2014
How to Cite
Plackett, A. R. G., Ferguson, A. C., Powers, S. J., Wanchoo-Kohli, A., Phillips, A. L., Wilson, Z. A., Hedden, P. and Thomas, S. G. (2014), DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis. New Phytologist, 201: 825–836. doi: 10.1111/nph.12571
- Issue published online: 9 JAN 2014
- Article first published online: 1 NOV 2013
- Manuscript Accepted: 15 SEP 2013
- Manuscript Received: 13 MAY 2013
- Rothamsted quota studentship
- Biotechnology and Biological Sciences Research Council of the UK. Grant Numbers: P16508, BB/J001295/1
- Arabidopsis thaliana ;
- DELLA ;
- ecotypic differences;
- male sterility;
- pollen development
- Excessive gibberellin (GA) signalling, mediated through the DELLA proteins, has a negative impact on plant fertility. Loss of DELLA activity in the monocot rice (Oryza sativa) causes complete male sterility, but not in the dicot model Arabidopsis (Arabidopsis thaliana) ecotype Landsberg erecta (Ler), in which DELLA function has been studied most extensively, leading to the assumption that DELLA activity is not essential for Arabidopsis pollen development. A novel DELLA fertility phenotype was identified in the Columbia (Col-0) ecotype that necessitates re-evaluation of the general conclusions drawn from Ler.
- Fertility phenotypes were compared between the Col-0 and Ler ecotypes under conditions of chemical and genetic GA overdose, including mutants in both ecotypes lacking the DELLA paralogues REPRESSOR OF ga1-3 (RGA) and GA INSENSITIVE (GAI).
- Ler displays a less severe fertility phenotype than Col-0 under GA treatment. Col-0 rga gai mutants, in contrast with the equivalent Ler phenotype, were entirely male sterile, caused by post-meiotic defects in pollen development, which were rescued by the reintroduction of DELLA into either the tapetum or developing pollen.
- We conclude that DELLA activity is essential for Arabidopsis pollen development. Differences between the fertility responses of Col-0 and Ler might be caused by differences in downstream signalling pathways or altered DELLA expression.