DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis
Article first published online: 1 NOV 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 201, Issue 3, pages 825–836, February 2014
How to Cite
Plackett, A. R. G., Ferguson, A. C., Powers, S. J., Wanchoo-Kohli, A., Phillips, A. L., Wilson, Z. A., Hedden, P. and Thomas, S. G. (2014), DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis. New Phytologist, 201: 825–836. doi: 10.1111/nph.12571
- Issue published online: 9 JAN 2014
- Article first published online: 1 NOV 2013
- Manuscript Accepted: 15 SEP 2013
- Manuscript Received: 13 MAY 2013
- Rothamsted quota studentship
- Biotechnology and Biological Sciences Research Council of the UK. Grant Numbers: P16508, BB/J001295/1
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Fig. S1 Loss of REPRESSOR OF ga1-3 (RGA) and GIBBERELLIN INSENSITIVE (GAI) causes infertility in the Col-0 background.
Fig. S2 Effect of gibberellin (GA) treatment on silique set and floral organ growth.
Fig. S3 Comparison of gid1a-1 gid1b-1 gid1c-1 and gid1a-1 gid1b-1 gid1c-2 phenotypes.
Fig. S4 The Ler DELLA global mutant retains pollen viability.
Fig. S5 Loss of ERECTA (ER) in the rga-28 gai-td1 (Col-0) background phenocopies the Ler growth habit.
Fig. S6 Additional anther and pollen phenotypes of rga-28 gai-td1.
Fig. S7 Reverse transcription-polymerase chain reaction (RT-PCR) analysis of REPRESSOR OF ga1-3-green fluorescent protein (RGA-GFP) expression in LTP12::RGA::GFP and LAT52::RGA::GFP transgenic lines.
Fig. S8 Vegetative and reproductive phenotypes of LTP12::RGA::GFP and LAT52::RGA::GFP transgenic lines.
Fig. S9 Fluorescence analysis of LTP12::RGA::GFP and LAT52::RGA::GFP expression.
Fig. S10 Genetic analysis of rga gai gid1 vegetative phenotypes.
Table S1 PCR primer sequences
Table S2 Col-0 and Ler respond differently to chemical and genetic gibberellin (GA) overdose
Table S3 Modelling the effect of gibberellin (GA) treatment on floral organ growth in wild-type Col-0
Table S4 Expression of gibberellin (GA) biosynthetic and signalling genes during wild-type Ler pollen development
Methods S1 Non-linear modelling of floral organ growth.