These authors contributed equally to this work.
The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria
Article first published online: 11 NOV 2013
No claim to original European Union works. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 4, pages 1371–1384, March 2014
How to Cite
Trdá, L., Fernandez, O., Boutrot, F., Héloir, M.-C., Kelloniemi, J., Daire, X., Adrian, M., Clément, C., Zipfel, C., Dorey, S. and Poinssot, B. (2014), The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria. New Phytologist, 201: 1371–1384. doi: 10.1111/nph.12592
- Issue published online: 3 FEB 2014
- Article first published online: 11 NOV 2013
- Manuscript Accepted: 6 OCT 2013
- Manuscript Received: 5 AUG 2013
- Agence Nationale de la Recherche and Comité National des Interprofessions des Vins d'appelation d'origine. Grant Number: 08-GENO-148G
- Conseil Régional de Bourgogne and Bureau Interprofessionnel des Vins de Bourgogne
- Region Champagne-Ardenne
- INTERREG IV program France-Wallonie-Vlaanderen
- The Gatsby Charitable Foundation
- (BBSRC) . Grant Number: BB/G024936/1
Please note: Wiley Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 flg22 triggers a dose-dependent oxidative burst in grapevine.
Fig. S2 Confirmation of the plasma membrane localization of VvFLS2.
Fig. S3 Burkholderia phytofirmans living bacteria, crude extract or purified flagellin triggers grapevine and Arabidopsis immunity.
Fig. S4 flg22 epitopes from different bacteria induce differential gene expression and growth inhibition in grapevine.
Fig. S5 Bp flg22, Pa flg22 and Xc flg22 induce a similar defense gene expression in Arabidopsis.
Fig. S6 Burkholderia phytofirmans overcomes Xc flg22-induced microbe-associated molecular pattern-triggered immunity (MTI) to colonize grapevine plantlets.
Table S1 Percentage of amino acid identity or similarity between AtFLS2, LeFLS2, OsFLS2 and VvFLS2
Table S2 Growth inhibition triggered by the different flg22 epitopes in Arabidopsis thaliana (wild-type (WT) Col-0 and the complemented line fls2/p35S::VvFLS2 #3) and Vitis vinifera (WT Chardonnay)
Methods S1 Histochemical β-glucuronidase (GUS) detection in Arabidopsis pPR1::GUS seedlings.
Methods S2 Bacterial inoculum and plant infection.