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Fig. S1 Distribution of 1Cx values found across Melanthiaceae and representative fluorescence histograms of the genome size assessment.

Fig. S2 Ancestral state reconstruction conducted under maximum likelihood (ML), and ancestral state reconstruction conducted under maximum parsimony (MP).

Fig. S3 Ancestral chromosome numbers inferred under maximum likelihood (ML) optimization.

Fig. S4 Karyotypes and idiograms of taxa of Parideae.

Table S1 Species included in the present study, chromosome numbers and GenBank/EMBL accession numbers

Table S2 Model fitness scores obtained with ChromEvol