RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

Authors

  • Marie Randoux,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
    2. Agrocampus Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-OUEST, Université d'Angers), SFR 4207 QUASAV, Angers, France
    3. Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), Université d'Angers, SFR 4207 QUASAV, PRES LUNAM, Beaucouzé Cedex, France
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  • Jean-Michel Davière,

    1. Unité Propre de Recherche 2357, CNRS, Institut de Biologie Moléculaire des Plantes, Conventionné avec l'Université de Strasbourg, Strasbourg Cedex, France
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  • Julien Jeauffre,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Tatiana Thouroude,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Sandrine Pierre,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Youness Toualbia,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Justine Perrotte,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Jean-Paul Reynoird,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
    2. DNM Plant Breeding, Institut Polytechnique - LaSalle Beauvais, Beauvais, France
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  • Marie-José Jammes,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Laurence Hibrand-Saint Oyant,

    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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  • Fabrice Foucher

    Corresponding author
    1. INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, Beaucouzé Cedex, France
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Summary

  • FT/TFL1 family members have been known to be involved in the development and flowering in plants. In rose, RoKSN, a TFL1 homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate RoKSN and to explore its mode of action in rose.
  • We complemented Arabidopsis tfl1 mutants and ectopically expressed RoKSN in a continuous-flowering (CF) rose. Using different protein interaction techniques, we studied RoKSN interactions with RoFD and RoFT and possible competition.
  • In Arabidopsis, RoKSN complemented the tfl1 mutant by rescuing late flowering and indeterminate growth. In CF roses, the ectopic expression of RoKSN led to the absence of flowering. Different branching patterns were observed and some transgenic plants had an increased number of leaflets per leaf. In these transgenic roses, floral activator transcripts decreased. Furthermore, RoKSN was able to interact both with RoFD and the floral activator, RoFT. Protein interaction experiments revealed that RoKSN and RoFT could compete with RoFD for repression and activation of blooming, respectively.
  • We conclude that RoKSN is a floral repressor and is also involved in the vegetative development of rose. RoKSN forms a complex with RoFD and could compete with RoFT for repression of flowering.

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