These authors contributed equally to this work.
The symbiotic transcription factor MtEFD and cytokinins are positively acting in the Medicago truncatula and Ralstonia solanacearum pathogenic interaction
Article first published online: 11 DEC 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 4, pages 1343–1357, March 2014
How to Cite
Moreau, S., Fromentin, J., Vailleau, F., Vernié, T., Huguet, S., Balzergue, S., Frugier, F., Gamas, P. and Jardinaud, M.-F. (2014), The symbiotic transcription factor MtEFD and cytokinins are positively acting in the Medicago truncatula and Ralstonia solanacearum pathogenic interaction. New Phytologist, 201: 1343–1357. doi: 10.1111/nph.12636
- Issue published online: 3 FEB 2014
- Article first published online: 11 DEC 2013
- Manuscript Accepted: 3 NOV 2013
- Manuscript Received: 26 JUL 2013
- Agence Nationale de la Recherche. Grant Number: ANR-08-PXXX-0-03
- Medicago truncatula ;
- pathogenesis–symbiosis crosstalk;
- Ralstonia solanacearum ;
- root pathogen;
- Sinorhizobium meliloti ;
- A plant–microbe dual biological system was set up involving the model legume Medicago truncatula and two bacteria, the soil-borne root pathogen Ralstonia solanacearum and the beneficial symbiont Sinorhizobium meliloti.
- Comparison of transcriptomes under symbiotic and pathogenic conditions highlighted the transcription factor MtEFD (Ethylene response Factor required for nodule Differentiation) as being upregulated in both interactions, together with a set of cytokinin-related transcripts involved in metabolism, signaling and response. MtRR4 (Response Regulator), a cytokinin primary response gene negatively regulating cytokinin signaling and known as a target of MtEFD in nodulation processes, was retrieved in this set of transcripts.
- Refined studies of MtEFD and MtRR4 expression during M. truncatula and R. solanacearum interaction indicated differential kinetics of induction and requirement of central regulators of bacterial pathogenicity, HrpG and HrpB. Similar to MtRR4, MtEFD upregulation during the pathogenic interaction was dependent on cytokinin perception mediated by the MtCRE1 (Cytokinin REsponse 1) receptor.
- The use of M. truncatula efd-1 and cre1-1 mutants evidenced MtEFD and cytokinin perception as positive factors for bacterial wilt development. These factors therefore play an important role in both root nodulation and root disease development.