Fine-scale diversity and distribution of ectomycorrhizal fungal mycelium in a Scots pine forest
Article first published online: 18 DEC 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 201, Issue 4, pages 1423–1430, March 2014
How to Cite
Anderson, I. C., Genney, D. R. and Alexander, I. J. (2014), Fine-scale diversity and distribution of ectomycorrhizal fungal mycelium in a Scots pine forest. New Phytologist, 201: 1423–1430. doi: 10.1111/nph.12637
- Issue published online: 3 FEB 2014
- Article first published online: 18 DEC 2013
- Manuscript Accepted: 6 NOV 2013
- Manuscript Received: 29 AUG 2013
- Natural Environment Research Council. Grant Number: NER/A/S/2002/00861
- Scottish Government
- ectomycorrhizal (ECM) mycelium;
- Scots pine (Pinus sylvestris);
- spatial ecology;
- terminal restriction fragment length polymorphism (T-RFLP)
- Ectomycorrhizal (ECM) mycelium is a key component of the ectomycorrhizal symbiosis, yet we know little regarding the fine-scale diversity and distribution of mycelium in ECM fungal communities.
- We collected four 20 × 20 × 2-cm3 (800-cm3) slices of Scots pine (Pinus sylvestris) forest soil and divided each into 100 2 × 2 × 2-cm3 (8-cm3) cubes. The presence of mycelium of ECM fungi was determined using an internal transcribed spacer (ITS) database terminal restriction fragment length polymorphism (T-RFLP) approach.
- As expected, many more ECM fungi were detected as mycelium than as ectomycorrhizas in a cube or slice. More surprisingly, up to one-quarter of the 43 species previously detected as ectomycorrhizas over an area of 400 m2 could be detected in a single 8-cm3 cube, and up to three-quarters in a single 800-cm3 slice. ECM mycelium frequency decreased markedly with depth and there were distinct ‘hotspots’ of mycelium in the moss/F1 layer.
- Our data demonstrate a high diversity of ECM mycelium in a small (8-cm3) volume of substrate, and indicate that the spatial scale at which ECM species are distributed as mycelium may be very different from the spatial scale at which they are distributed as tips.