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Fig. S1 Molecular phylogenetic analysis of ectomycorrhizal (ECM) fungal root tip samples of Lactarius obscuratus using the maximum likelihood method based on the Jukes–Cantor model.

Fig. S2 Nonmetric Multidimensional Scaling (NMDS) ordination of ectomycorrhizal (ECM) fungal community presence–absence on Alnus rubra and Pseudotsuga menziesii at Cascade Head and H.J. Andrews.

Fig. S3 Differences in potential enzyme activity between ectomycorrhizal (ECM) fungal communities at Cascade Head and H.J. Andrews in spring and summer.

Table S1 Best database matches and accession numbers for representative query sequences of all ectomycorrhizal fungi identified at Cascade Head and H.J. Andrews

Table S2 Permutational MANOVA (Permanova) of ectomycorrhizal (ECM) fungal community composition based on presence–absence per sample (core), with plot nested in season, site and host, and based on Bray–Curtis distances

Table S3 Permutational MANOVA (Permanova) of ectomycorrhizal (ECM) fungal community composition based on presence–absence per plot and Bray–Curtis distances

Table S4 Ectomycorrhizal (ECM) fungal species present in at least 5% of cores sampled per host on Alnus rubra and Pseudotsuga menziesii at Cascade Head and H.J. Andrews

Table S5 Univariate ANOVAs of ectomycorrhizal (ECM) fungal community potential enzyme activity at Cascade Head and H.J. Andrews on A. rubra and P. menziesii