SEARCH

SEARCH BY CITATION

Keywords:

  • abscisic acid;
  • cucurbits;
  • exudates;
  • flg22;
  • jasmonates;
  • phloem/xylemphytohormones;
  • plant defence;
  • salicylic acid

Summary

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References
  • The stress-related phytohormones, salicylic acid (SA) and abscisic acid (ABA), and the three jasmonates, jasmonic acid (JA), cis-12-oxo-phytodienoic acid (cis-OPDA), and (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), were investigated in phloem and xylem exudates of Cucurbita maxima.
  • Phloem and xylem exudates were separately collected and analysed via liquid chromatography–mass spectrometry.
  • We show direct evidence for all three jasmonates, ABA, and SA in both phloem and xylem exudates of C. maxima. JA and JA-Ile concentrations are higher in xylem (JA: cxylem ≈ 199.5 nM, cphloem ≈ 43.9 nM; JA-Ile: cxylem ≈ 7.9 nM, cphloem ≈ 1.6 nM), whereas ABA and SA concentrations are higher in phloem exudates (ABA: cxylem ≈ 37.1 nM, cphloem ≈ 142.6 nM; SA: cxylem ≈ 61.6 nM, cphloem ≈ 1319 nM). During bacteria-derived flagellin 22 (flg22)-triggered remote root-to-shoot signalling, phytohormone concentration changed rapidly both in phloem and xylem.
  • The unequal distribution of phytohormones suggests that phloem and xylem have distinct roles in defence responses. Our data shed light on systemic phytohormone signalling and help explain how plants cope with environmental challenges by lateral exchange between phloem and xylem. Our analysis is a starting point for further investigations of how phytohormones contribute to phloem- and xylem-based defence signalling.

Introduction

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References

Various phytohormones have been identified as important components of signalling cascades in plant development and plant response to various (a) biotic challenges (Beckers & Spoel, 2005; Pozo et al., 2005; Robert-Seilaniantz et al., 2011; Pieterse et al., 2012). Abscisic acid (ABA) is a well-known root-to-shoot signal produced in response to abiotic stress in the form of drought, heat, cold, salt or heavy metals (Christmann et al., 2006; Zhang et al., 2008), though it is also involved in the stress response to microbes (De Vleesschauwer et al., 2010). Salicylic acid (SA) and its derivative, methyl salicylate, are key signals in systemic acquired resistance (SAR) and the hypersensitive response (HR); both are typically involved in the control of biotrophic pathogens (Métraux et al., 1990; Vlot et al., 2009; Dempsey et al., 2011). The three jasmonates, cis-12-oxo-phytodienoic acid (cis-OPDA), jasmonic acid (JA), and (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), have been discussed as general intercellular and intracellular signalling compounds typically involved in multiple defence reactions to both necrotrophic microbial pathogens and herbivore attack (Verhage et al., 2011; Pel & Pieterse, 2013; Wasternack & Hause, 2013). However, JA-Ile has been identified as the proposed bioactive form of jasmonates that interacts with the corresponding receptor complex SCFCOI1 (Fonseca et al., 2009). The cross-talk between interacting hormone signalling cascades makes it necessary to consider stress-related phytohormones simultaneously (Engelberth et al., 2001; Koornneef & Pieterse, 2008; Diezel et al., 2009; Robert-Seilaniantz et al., 2011; Verhage et al., 2011).

Although many studies have emphasized the role of jasmonates, SA derivates, and ABA in plant defences, the nature of the long-distance signal(s) is still controversial (Dempsey & Klessig, 2012; Matsuura et al., 2012), at least in part because little is known about the distinct role of the translocation pathway – the vascular system.

The vascular system of higher plants pervades the plant from roots to leaf tips and ensures communication and nutrient supply among all plant organs and tissues. The balance between phloem and xylem guaranties the reliability of the short- and long-distance transport of various constituents, such as carbohydrates, proteins, inorganic ions, water, and secondary metabolites (van Bel et al., 2011; Lucas et al., 2013). Hence, the analysis of the composition of phloem and xylem sap offers crucial information about a plant's physiological status (Dinant et al., 2010). Although most studies have observed amino acids, proteins, (in)organic ions and carbohydrates (e.g. Richardson et al., 1982; Shelp, 1987) in the vascular sap, few have quantitatively analysed phytohormones (see Table 1). The importance of phloem and xylem as tubes for propagating phytohormones has often been suggested but is not well understood.

Table 1. Reported concentrations of phytohormones in phloem and xylem exudates
PhytohormonePhloemXylem
c (nM)SpeciesReferencec (nM)SpeciesReferences
  1. Note: JA, jasmonic acid; JA-Ile, (+)-7-iso-jasmonoyl-l-isoleucine; SA, salicylic acid; ABA, abscisic acid.

  2. Some values are converted to molar (c = nM) specification.

  3. a

    Phloem sap was collected using stylectomy.

JA   170–500 Brassica napus Ratzinger et al. (2009)
   70 Solanum lycopersicum Matsuura et al. (2012)
JA–Ile   180 S. lycopersicum Matsuura et al.(2012)
SA0–10 Cucurbita maxima Métraux et al. (1990)100–180 Brassica napus Ratzinger et al. (2009)
ABA200–500 Ricinus communis Zhong et al. (1996)260 R. communis Zhong et al. (1996)
1800 R. communis Jeschke et al. (1997)180 R. communis Jeschke et al. (1997)
4000 R. communis Else et al. (2001)220 R. communis Else et al. (2001)
90 R. communis Peuke et al. (2002)1700 R. communis Peuke et al. (2002)
650–790a Oryza sativa Yokota et al. (1994)60–100 Cucurbita maxima Zhang et al. (2008)
   40–75 Cucurbita ficifolia Zhang et al. (2008)
   60–140 Cucumis sativus Zhang et al. (2008)
   50–95 Cucumis melo Zhang et al. (2008)
   75–80 Momordica charantia Zhang et al. (2008)
   75–100 Benincasa hispida Zhang et al. (2008)
   2–42 Brassica napus Ratzinger et al. (2009)
   and many more data available for Helianthus annuus, Lupinus albus, Lycopersicon esculentum and Zea mays

Here we individually analyse the presence of stress-related phytohormones in phloem and xylem exudates and in response to challenge with the bacterial flagellin 22 (flg22).

Materials and Methods

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References

Plant material

The growth conditions for Cucurbita maxima Duch. plants and sampling of phloem and xylem exudates have been described (see Zimmermann et al., 2013; also see Fig. 1). Cucurbita maxima plants (cv GeleReuzen; Enza Zaden, Enkhuizen, the Netherlands) were cultivated in pots in a glasshouse under standard conditions (21°C, 60–70% relative humidity, and a 14 h : 10 h, light : dark period). Plants were taken for experiments 21–24 d after germination. Cuts were placed at the stem 10–20 mm above soil.

image

Figure 1. Sampling procedure of phloem and xylem exudates in Cucurbita maxima. (a) Jutting roots are incubated in tap water containing 1.0 μM flagellin 22 (flg22) for 10, 30, or 180 min. (b) The stem is cut 10–20 mm above the soil level. The droplets exuded from both cut sides (apical and basal) within the first minute (< 2 min) are collected and represent diluted phloem sap. To achieve a high volume of phloem exudates, the apical side can be cut 1–3 times. (c) To harvest xylem exudate, the remaining gelled droplets of phloem exudate on the wound surface of the basal side are removed with a fluffless cloth after 2 min at the earliest (> 2 min). (d) The subsequent exudates represent xylem exudates; they are monitored for the characteristic pH-value (Zimmermann et al., 2013). Volumes of phloem and xylem exudates ranged between 90 and 210 μl per plant. Plants were used 21–24 d after germination.

Download figure to PowerPoint

Phytohormone determination

To concentrate samples, 3 ml of either exudate was first acidified by adding 15 μl formic acid (0.5% v/v final concentration) to a mix of internal phytohormone standards (Vadassery et al., 2012). Subsequently, the sample was loaded onto a reversed-phase column (Oasis HLB 3cc; Waters, Eschborn, Germany) that was first pre-conditioned by the addition of 2 ml methanol (MeOH) followed by 2 ml water. After being washed with 2 ml 5% (v/v)MeOH/water solution containing 0.5% (v/v) formic acid, the column was eluted with 2 ml MeOH. The eluate was completely dried in a speed-vac, resolved in 0.1 ml MeOH and subsequently subjected to liquid chromatography–mass spectrometry analysis according to Vadassery et al. (2012).

Application of stimulus

Flagellin 22 (flg22; Davids Biotechnologie GmbH, Regensburg, Germany) was applied to the root system with a final concentration of 1 μM (Fig. 1). Afterwards, samples of phloem and xylem exudates were collected at 10, 30 and 180 min after flg22 treatment. The distance between the stimulus site at the root and the sampling location at the stem was c. 70–80 mm.

Statistical analyses

Statistical analyses were carried out using one-way analysis of variance (ANOVA) and post hoc test (Tukey; SigmaStat® 3.0; SPSS Inc., Chicago, IL, USA). The statistical significance level was set to 5% (< 0.05).

Results and Discussion

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References

A main reason for the lack of integrated knowledge about phytohormones in the vasculature is the difficulty in obtaining unimpeded access to vascular sap. Cucurbits spontaneously exude vascular sap from the wound surface. This characteristic allows both phloem and xylem exudates to be sampled separately from the same site of an individual plant, which is a prerequisite for a comparative quantitative analysis (Zimmermann et al., 2013). Thus, we used Cucurbita maxima as a model to study the distribution of phytohormones in xylem and phloem and, in addition, defence-related alterations in the vascular sap in more detail.

All considered metabolites – the three jasmonates, cis-OPDA, JA, JA-Ile, as well as SA and ABA – were detected in the exudates of both phloem and xylem (Fig. 2), and significant (ANOVA, < 0.05) differences among the composition of phloem and xylem exudates were assessed. However, it has recently been found that the process of exudation forces out the content of phloem by an osmotically driven lateral water influx from the xylem/apoplast into the sieve elements (SEs; Zimmermann et al., 2013). As a consequence, the collected phloem sap is diluted. Therefore, the measured values underestimate the in vivo situation by c. 7–8-fold. This factor was determined by carbohydrate measurements in exudates (Zimmermann et al., 2013). After the application of the dilution factor, a significant difference between phloem and xylem exudates remains for ABA, SA, JA and JA-Ile, whereas almost equal concentrations of cis-OPDA were calculated in phloem and xylem exudates (Fig. 2).

image

Figure 2. Analysis of phytohormones in phloem and xylem exudates of unstressed Cucurbita maxima plants. The concentrations (in nM) of the phytohormones cis-12-oxo-phytodienoic acid (cis-OPDA), jasmonic acid (JA), (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), salicylic acid (SA), and abscisic acid (ABA) are individually determined in phloem (black bars) and xylem (white bars) exudates from the same site of a stem-cut plant (nplant = 8). The dilution of phloem exudates with xylem water is considered with a factor of eight (grey bars) to respect the in vivo situation (Zimmermann et al., 2013). The statistical significance is tested with one-way ANOVA by pairwise comparison of xylem and phloem exudates (also phloem ×8) with Student′s t-test (< 0.05) and marked with different letters (superscript apostrophe for phloem ×8). Vertical lines represent standard deviations of eight replicates.

Download figure to PowerPoint

In light of the dilution, the higher concentration of ABA in the phloem exudates (c = 142.5 ± 65.3 nM) compared to the xylem exudates (c = 37.1 ± 16.1 nM) was unexpected. ABA is a root-borne signal that is released to the above-ground plant part (Christmann et al., 2006; Zhang et al., 2008). Recently it was shown that ABA is exported from the producing cells in the roots into the xylem by the ABC-transporter AtABCG25 (Kuromori et al., 2010). Hence, the xylem should be the most likely translocation pathway. Consistent with our results, however, previous comparative studies have reported higher ABA concentrations in the phloem exudates of Ricinus communis (Table 1; Jeschke et al., 1997; Else et al., 2001). ABA in the phloem was suggested to be loaded within leaves and earlier was proposed to act as a reservoir (Wilkinson & Davies, 2002). Despite abundant surveys of ABA in the vascular sap, fundamental knowledge is lacking about how ABA is distributed between the phloem and xylem in unstressed plants (Table 1; Zhong et al., 1996; Peuke et al., 2002).

Substantial differences between phloem and xylem exudates were found for SA and JA independent of the effect of dilution, suggesting that each has a preferred translocation pathway (Fig. 2). A higher concentration of SA was found in phloem exudates (1.5-fold) than in xylem exudates (cxylem = 61.6 ± 17.2 nM, cphloem = 165 ± 60.6 nM); a higher concentration of SA has also been reported for Ricinus communis (Rocher et al., 2006). The content of SA in vivo is even higher (cphloem = 1319 ± 484 nM) when the dilution factor is taken into account. For unknown reasons, Métraux et al. (1990) observed low SA concentrations (c. 0–10 nM) in the phloem exudates of unstressed C. maxima plants, while concentrations in R. communis were at least 20 times higher. Unlike SA, the highest amounts of JA by far (36-fold) were detected in xylem exudates (cxylem = 199.5 ± 71.5 nM, cphloem = 5.5 ± 1.9 nM), which confirms other data available (Ratzinger et al., 2009). Further details about the distribution of JA within phloem and xylem are not available yet.

At first glance, the different distribution patterns of JA, SA and ABA in phloem and xylem exudates are surprising: JA (pKa = 4.5), SA (pKa = 3.02) and ABA (pKa = 4.87) are weak organic acids and thus share similar physico-chemical properties. Any enrichment of these compounds within the SEs/phloem would have been expected due to the ion trap mechanism (Delrot et al., 1981; Kramer, 2006; Rocher et al., 2006). The protonated forms of JA, SA and ABA can permeate membranes and are supported more by the weak acidic environment of the xylem (pH = 4.8–5.5; Crafts, 1936; Richardson et al., 1982; Zimmermann et al., 2013) than by the alkaline/neutral environment of the SEs/phloem (pH = 7.5–8.0; Crafts, 1936; Richardson et al., 1982; Zimmermann et al., 2013). However, it is worth to mention that at pH 5.0 in particular JA and also cis-OPDA are predicted to be mainly in their poorly permeant dissociated form. Within the xylem/apoplast, the balance between protonated and de-protonated forms is shifted to the protonated one. Hence, there may be a net influx from the xylem/apoplast into the SEs/phloem. Inside the SEs/phloem, JA, SA and ABA are immediately de-protonated, and the nonmembrane-permeable anion is trapped by the SEs/phloem; the consequence is an increase of JA, SA and ABA. Indeed, the high level of SA in the phloem exudate of Ricinus seedlings was previously explained by the combination of the ion trap mechanism and a postulated specific carrier system (Rocher et al., 2006, 2009). The higher concentration of ABA in the phloem may also be assisted by a trans-membrane translocation system as described for xylem loading in Arabidopsis (Kuromori et al., 2010; Kang et al., 2011). However, such importer/exporter that might be involved in phloem loading still needs to be identified.

Such a system is very likely due to the high permeability of ABA in SEs (Hartung et al., 2002). However, the findings for JA are unexpected, since, in addition to the ion trap mechanism, diverse enzymes (lipoxygenase, ACC-oxidase/synthase) involved in jasmonate biosynthesis were detected directly in phloem exudates (Avdiushko et al., 1994; Walz et al., 2004) and within SEs and the companion cells of tomato plants (Hause et al., 2000, 2003; Stenzel et al., 2003). Thus, it is tempting to speculate that JA, like SA, accumulates in the phloem and is actively exported from the phloem into the xylem. Whereas a study using exogenously applied carbon-14 (14C)-labelled JA suggested a transport from leaves to roots in Nicotiana sylvestris (Zhang & Baldwin, 1997), grafting experiments with jasmonate insensitive and jasmonate biosynthesis mutants, respectively, showed that a root to shoot direction for jasmonates is likely (Howe, 2004). Consistent with these later results, more recent studies reported a synthesis of JA in the root from where it is translocated to the shoot via the xylem (López-Ráez et al., 2010). However, the mechanism by which the protonated JA has to be prevented from refluxing is still unknown.

The absence of literature that refers to direct JA measurements in the vascular sap motivated us to investigate the related metabolites cis-OPDA and JA-Ile. cis-OPDA, the biosynthetic precursor of JA, has diverse signalling functions. Recently, inhibition of seed germination and interaction with ABA was reported (Dave & Graham, 2012). Unlike JA and JA-Ile, cis-OPDA did not accumulate preferentially in the xylem exudates when the dilution factor is taken into account (cxylem = 48.6 ± 25.5 nM, cphloem = 29 ± 16.3 nM), raising the possibility for the presence of an independent distribution. The conjugate JA-Ile (Fig. 2, inset) is a highly active wound-response signal (Fonseca et al., 2009; Matsuura et al., 2012). Notably, the highest relative differences (40-fold and five-fold, respectively, when dilution is included) between phloem and xylem exudates were observed for JA-Ile (cxylem = 7.9 ± 5 nM, cphloem = 0.2 ± 0.06 nM). This finding supports as well the hypothesis of a preferred translocation pathway for certain phytohormones. The observed low concentrations of JA-Ile in comparison to JA and cis-OPDA may be indicative of a highly efficient signal. A previous study of S. lycopersicum demonstrated much higher amounts of JA-Ile in the xylem (18-fold) in comparison to our results, and the concentration of JA-Ile was found to be twice as high as that of JA (Table 1; Matsuura et al., 2012). The detection of methyl-jasmonate and methyl-salicylate in vascular exudates would strongly amplify our knowledge; however, an advancement of the sampling procedure is a precondition due to the volatile characteristic and will be a challenge of future work.

The different lateral distribution patterns of the jasmonates, SA and ABA, raise questions about the role of phloem and xylem during signalling. No previous studies have proposed an adequate explanation of the imbalanced distributions. Now that it is possible to determine the concentrations of various phytohormones in parallel in xylem and phloem independently, studies addressing the role and physiology of these signalling compounds under different stress situations can be performed. Because phloem and xylem are known to interact in order to ensure the translocation of photoassimilates, they should be seen as a functional unit collaborating during short- and long-distance phytohormone signalling.

We assessed collaboration between phloem and xylem in stress-related root-to-shoot signalling after the application of 1 μM flg22 to roots (see Fig. 1 for methods). It has been reported that binding of bacteria-derived flg22 to the plant's transmembrane receptor kinase FLAGELLIN SENSITIVE2 (FLS2) induces innate immune responses that affect the defence-related phytohormone homeostasis (Nürnberger & Brunner, 2002; Chinchilla et al., 2006, 2007; Boller & Felix, 2009).

As shown in Figure 3, significant (ANOVA, < 0.05) flg22-triggered remote effects were detected for all examined phytohormones in both phloem and xylem after flg22-application to roots; this is consistent with a role of phytohormones in immune signalling (Engelberth et al., 2001; Flors et al., 2007; Koornneef & Pieterse, 2008; Diezel et al., 2009; Dempsey & Klessig, 2012; Pieterse et al., 2012). We observed significant short-term responses, especially for jasmonates and SA, after 10–180 min, which complemented previous long-term studies that reported such responses for time ranges between 0.5 and 3 d following an application of Pseudomonas syringae, tobacco necrosis virus or the fungal pathogen Colletotrichum lagenarium (Métraux et al., 1990; Rasmussen et al., 1991; Smith-Becker et al., 1998). Also, short-term effects of phloem-located JA and JA-Ile have been recently observed after mechanical wounding of Cucurbita maxima leaf edges (Gaupels et al., 2012).

image

Figure 3. Analysis of phytohormones in phloem and xylem exudates of flagellin 22 (flg22)-treated Cucurbita maxima plants. Distinct xylem (white bars) and phloem (black bars) exudates are separately collected at the stem cut 10, 30 or 180 min after incubation of Cucurbita maxima roots in 1 μM flg22. The dilution of phloem exudates is considered with a factor of eight (grey bars) to represent the in vivo situation (Zimmermann et al., 2013). Time point 0 min represents untreated plants. The concentrations (c = nM) of jasmonic acid (JA), (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), cis-12-oxo-phytodienoic acid (cis-OPDA), salicylic acid (SA), and abscisic acid (ABA) are directly determined in phloem and xylem exudates (a–e). Each bar represents the mean of 7–15 replicates (plants). Vertical lines, ± standard deviation (SD). The statistical significance is tested with one-way ANOVA (post hoc test: Tukey; < 0.05) and marked with asterisks. Note that the illustrated significance compares untreated plants (= 0 min) with the different flg22 time points (10, 30 and 180 min) separate for xylem and phloem exudates.

Download figure to PowerPoint

The present findings of simultaneous phytohormone alterations suggest that xylem and phloem interact. Inside the phloem, the reaction pattern of SA and ABA in response to flg22 treatment was unlike the reaction pattern of jasmonates (Fig. 3a–e). Initially, levels of SA (−43.6%) and ABA (−46.5%) significantly decreased until 180 min, whereas levels of jasmonates significantly increased until 180 min. The findings of anticyclical pattern of SA and jasmonates may confirm previous results and may indicate that an innate immunity response has been initiated (Flors et al., 2007; Koornneef & Pieterse, 2008; Diezel et al., 2009; Vlot et al., 2009). The apparent suppression of JA in the xylem may also be explained by a lateral exchange. The significant increase of JA in the phloem (+89.7%) and the simultaneous decrease of JA in the xylem (−89%) indicates that a lateral exchange has occurred. This conclusion also is supported by the significant decline of cis-OPDA in the xylem (−74.5%) and the coincidental rise of cis-OPDA in the phloem (+31%; Fig. 3c). The xylem-originated cis-OPDA might serve as substrate for the essential enzymes of JA biosynthesis detected in vascular parenchyma cells, companion cells and SEs (Hause et al., 2000, 2003; Stenzel et al., 2003). Presumably de novo synthesized JA is translocated from cell to cell via plasmodesmata into SEs. It is very interesting that the lateral distribution of jasmonates between phloem and xylem in untreated plants (Fig. 2) significantly changed after 30 min of flg22 application (Fig. 3a) or, rather, completely reversed (cis-OPDA), when the dilution of phloem exudates was considered, and indicated additionally a lateral exchange. However, besides lateral exchanges as part of allocation processes (Schultz et al., 2013), it is also tempting to speculate that the particular stress factor, flg22, has a negative effect on xylem loading of jasmonates. Other biotic challenges such as herbivory or different pathogens should be employed in future studies to address this question.

The lateral exchange of phytohormones between xylem and phloem during plant defence signalling suggests that the process of long-distance signalling is more inclusive than the longitudinal delivery of a signalling constituent. Future studies should focus on the molecular mechanisms by which phloem and xylem collaborate during short- and long-distance phytohormone signalling.

Acknowledgements

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References

This work was supported by the Justus Liebig University in the frame of ‘Just'us postdoc grant’, the ‘Margarete Bieber grant’, and the Max Planck Society. The authors thank E. Wheeler, Boston, for editorial assistance.

References

  1. Top of page
  2. Summary
  3. Introduction
  4. Materials and Methods
  5. Results and Discussion
  6. Acknowledgements
  7. References
  • Avdiushko SA, Ye XS, Kuc J, Hildebrand DF. 1994. Lipoxygenase is an abundant protein in cucumber exudates. Planta 193: 349357.
  • Beckers GJM, Spoel SH. 2005. Fine-tuning plant defence signalling: salicylate versus jasmonate. Plant Biology 8: 110.
  • van Bel AJE, Furch ACU, Hafke JB, Knoblauch M, Patrick JW. 2011. (Questions)n on phloem biology. 2. Mass flow, molecular hopping, distribution patterns and macromolecular signalling. Plant Science 181: 325330.
  • Boller T, Felix G. 2009. A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annual Review of Plant Biology 60: 379406.
  • Chinchilla D, Bauer Z, Regenass M, Boller T, Felix G. 2006. The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. The Plant Cell 18: 465476.
  • Chinchilla D, Zipfel C, Robatzek S, Kemmerling B, Nürnberger T, Jones JDG, Felix G, Boller T. 2007. A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature 448: 497500.
  • Christmann A, Moes D, Himmelbach A, Yang Y, Tang Y, Grill E. 2006. Integration of abscisic acid signalling into plant responses. Plant Biology 8: 314325.
  • Crafts AS. 1936. Further studies on exudation in cucurbits. Plant Physiology 11: 6379.
  • Dave A, Graham I. 2012. Oxylipin signaling: a distinct role for the jasmonic acid precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA). Frontiers in Plant Science 3: 42.
  • De Vleesschauwer D, Yang Y, Cruz CV, Höfte M. 2010. Abscisic acid-induced resistance against the brown spot pathogen Cochliobolus miyabeanus in rice involves MAP kinase-mediated repression of ethylene signaling. Plant Physiology 152: 20362052.
  • Delrot S, Everatbourbouloux A, Bonnemain JL. 1981. On the effect of pH on the uptake and the export of abscisic-acid by leaf tissues of Vicia-faba L. Comptes Rendus de l′Academie des Sciences Serie III-Sciences de la Vie-Life Sciences 293: 659663.
  • Dempsey DA, Vlot AC, Wildermuth MC, Klessig DF. 2011. Salicylic acid biosynthesis and metabolism. The Arabidopsis Book 9: e0156.
  • Dempsey MA, Klessig DF. 2012. SOS – too many signals for systemic acquired resistance? Trends in Plant Science 17: 538545.
  • Diezel C, von Dahl CC, Gaquerel E, Baldwin IT. 2009. Different lepidopteran elicitors account for cross-talk in herbivory-induced phytohormone signaling. Plant Physiology 150: 15761586.
  • Dinant S, Bonnemain J-L, Girousse C, Kehr J. 2010. Phloem sap intricacy and interplay with aphid feeding. Comptes Rendus Biologies 333: 504515.
  • Else MA, Coupland D, Dutton L, Jackson MB. 2001. Decreased root hydraulic conductivity reduces leaf water potential, initiates stomatal closure and slows leaf expansion in flooded plants of castor oil (Ricinus communis) despite diminished delivery of ABA from the roots to shoots in xylem sap. Physiologia Plantarum 111: 4654.
  • Engelberth J, Koch T, Schüler G, Bachmann N, Rechtenbach J, Boland W. 2001. Ion channel-forming alamethicin is a potent elicitor of volatile biosynthesis and tendril coiling. Cross talk between jasmonate and salicylate signaling in lima bean. Plant Physiology 125: 369377.
  • Flors V, Ton J, van Doorn R, Jakab G, García-Agustín P, Mauch-Mani B. 2007. Interplay between JA, SA and ABA signalling during basal and induced resistance against Pseudomonas syringae and Alternaria brassicicola. Plant Journal 54: 8192.
  • Fonseca S, Chini A, Hamberg M, Adie B, Porzel A, Kramell R, Miersch O, Wasternack C, Solano R. 2009. (+)-7-iso-Jasmonoyl-l-isoleucine is the endogenous bioactive jasmonate. Nature Chemical Biology 5: 344350.
  • Gaupels F, Sarioglu H, Beckmann M, Hause B, Spannagl M, Draper J, Lindermayr C, Durner J. 2012. Deciphering systemic wound responses of the pumpkin extra fascicular phloem by metabolomics and stable isotope-coded protein labeling. Plant Physiology 160: 22852299.
  • Hartung W, Sauter A, Hose E. 2002. Abscisic acid in the xylem: where does it come from, where does it goes? Journal of Experimental Botany 53: 2732.
  • Hause B, Hause G, Kutter C, Miersch O, Wasternack C. 2003. Enzymes of jasmonate biosynthesis occur in tomato sieve elements. Plant and Cell Physiology 44: 643648.
  • Hause B, Stenzel I, Miersch O, Maucher H, Kramell R, Ziegler J, Wasternack C. 2000. Tissue-specific oxylipin signature of tomato flowers: allene oxide cyclase is highly expressed in distinct flower organs and vascular bundles. Plant Journal 24: 113126.
  • Howe GA. 2004. Jasmonates as signals in the wound response. Journal of Plant Growth Regulation 23: 223237.
  • Jeschke WD, Peuke AD, Pate JS, Hartung W. 1997. Transport, synthesis and catabolism of abscisic acid (ABA) in intact plants of castor bean (Ricinus communis L.) under phosphate deficiency and moderate salinity. Journal of Experimental Botany 48: 17371747.
  • Kang J, Park J, Choi H, Burla B, Kretzschmar T, Lee Y, Martinoia E. 2011. Plant ABC transporter. The Arabidopsis Book 9: e0153.
  • Koornneef A, Pieterse CMJ. 2008. Cross talk in defense signaling. Plant Physiology 146: 839844.
  • Kramer EM. 2006. How far can a molecule of weak acid travel in the apoplast or xylem?Plant Physiology 141: 12331236.
  • Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K. 2010. ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proceedings of the National Academy of Sciences, USA 107: 23612366.
  • López-Ráez JA, Verhage A, Fernández I, García JM, Azcón-Aguilar C, Flors V, Pozo MJ. 2010. Hormonal and transcriptional profiles highlight common and differential host responses to arbuscular mycorrhizal fungi and the regulation of the oxylipin pathway. Journal of Experimental Botany 61: 25892601.
  • Lucas WJ, Groover A, Lichtenberger R, Furuta K, Yadav S-R, Helariutta Y, He X-Q, Fukuda H, Kang J, Brady SM et al. 2013. The plant vascular system: evolution, development and functions. Journal of Integrative Plant Biology 55: 294388.
  • Matsuura H, Takeishi S, Kiatoka N, Sato C, Sueda K, Masuta C, Nabeta K. 2012. Transportation of de novo synthesized jasmonoyl isoleucine in tomato. Phytochemistry 83: 2533.
  • Métraux JP, Signer H, Ryals J, Ward E, Wyss-Benz M, Gaudin J, Raschdorf K, Schmid E, Blum W, Inverardi B. 1990. Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science 250: 10041006.
  • Nürnberger T, Brunner F. 2002. Innate immunity in plants and animals: emerging parallels between the recognition of general elicitors and pathogen-associated molecular patterns. Current Opinion in Plant Biology 5: 318324.
  • Pel MJC, Pieterse CMJ. 2013. Microbial recognition and evasion of host immunity. Journal of Experimental Botany 64: 12371248.
  • Peuke AD, Jeschke DW, Hartung W. 2002. Flows of elements, ions and abscisic acid in Ricinus communis and site of nitrate reduction under potassium limitation. Journal of Experimental Botany 53: 241250.
  • Pieterse CMJ, Van der Does D, Zamioudis C, Leon-Reyes A, Van Wees SCM. 2012. Hormonal modulation of plant immunity. Annual Review of Cell and Developmental Biology 28: 489521.
  • Pozo MJ, van Loon LC, Pieterse CMJ. 2005. Jasmonates – signals in plant–microbe interactions. Journal of Plant Growth and Regulation 23: 211222.
  • Rasmussen JB, Hammerschmidt R, Zook MN. 1991. Systemic induction of salicylic acid accumulation in cucumber after inoculation with Pseudomonas syringae pv syringae. Plant Physiology 97: 13421347.
  • Ratzinger A, Riediger N, von Tiedemann A, Karlovsky P. 2009. Salicylic acid and salicylic acid glucoside in xylem sap of Brassica napus infected with Verticillium longisporum. Journal of Plant Research 122: 571579.
  • Richardson PT, Baker DA, Ho LC. 1982. Chemical composition of cucurbit vascular exudates. Journal of Experimental Botany 33: 12391247.
  • Robert-Seilaniantz A, Grant M, Jones JDG. 2011. Hormone crosstalk in plant disease and defense: more than just JASMONATE-SALICYLATE antagonism. Annual Review of Phytopathology 49: 317343.
  • Rocher F, Chollet J-F, Jousse C, Bonnemain J-L. 2006. Salicylic acid, an ambimobile molecule exhibiting a high ability to accumulate in the phloem. Plant Physiology 141: 16841693.
  • Rocher F, Chollet J-F, Legros S, Jousse C, Lemoine R, Faucher M, Bush DR, Bonnemain J-L. 2009. Salicylic acid transport in Ricinus communis involves a pH-dependent carrier system in addition to diffusion. Plant Physiology 150: 20812091.
  • Schultz JC, Appel HM, Ferrieri AP, Arnold TM. 2013. Flexible resource allocation during plant defense responses. Frontiers in Plant Science 4: 324.
  • Shelp BJ. 1987. The composition of phloem exudate and xylem sap from broccoli (Brassica oleracea var. italica) supplied with NH4+, NO3 or NH4NO3. Journal of Experimental Botany 38: 16191636.
  • Smith-Becker J, Marois E, Huguet EJ, Midland SL, Sims JJ, Keen NT. 1998. Accumulation of salicylic acid and 4-hydroxybenzoic acid in phloem fluids of cucumber during systemic acquired resistance is preceded by a transient increase in phenylalanine ammonia-lyase activity in petioles and stems. Plant Physiology 116: 231238.
  • Stenzel I, Hause B, Maucher H, Pitzschke A, Miersch O, Ziegler J, Ryan CA, Wasternack C. 2003. Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato – amplification in wound signalling. Plant Journal 33: 577589.
  • Vadassery J, Reichelt M, Hause B, Gershenzon J, Boland W, Mithöfer A. 2012. CML42-mediated calcium signaling co-ordinates responses to Spodoptera herbivory and abiotic stresses in Arabidopsis. Plant Physiology 159: 11591175.
  • Verhage A, Vlaardingerbroek I, Raaymakers C, van Dam NM, Dicke M, van Wees SCM, Pieterse CMJ. 2011. Rewiring of the jasmonate signalling pathway in Arabidopsis during insect herbivory. Frontiers in Plant Science 2: 47.
  • Vlot AC, Dempsey MA, Klessig DF. 2009. Salicylic acid, a multifaceted hormone to combat disease. Annual Review of Phytopathology 47: 177206.
  • Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J. 2004. Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry 65: 17951804.
  • Wasternack C, Hause B. 2013. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. Annals of Botany 111: 10211058.
  • Wilkinson S, Davies WJ. 2002. ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant, Cell & Environment 25: 195210.
  • Yokota T, Nakayama M, Harasawa I, Sato M, Katsuhara M, Kawabe S. 1994. Polyamines, indole-3-acetic acid and abscisic acid in rice phloem sap. Plant and Growth Regulation 15: 125128.
  • Zhang YP, Qiao YX, Zhang YL, Zhou YH, Yu JQ. 2008. Effects of root temperature on leaf gas exchange and xylem sap abscisic acid concentrations in six cucurbitaceae species. Photosynthetica 46: 356362.
  • Zhang ZP, Baldwin IT. 1997. Transport of [2-C-14]jasmonic acid from leaves to roots mimics wound-induced changes in endogenous jasmonic acid pools in Nicotiana sylvestris. Planta 203: 436441.
  • Zhong W, Hartung W, Schobert C, Komor E. 1996. Phloem transport of abscisic acid in Ricinus communis L. seedlings. Plant, Cell & Environment 19: 471477.
  • Zimmermann MR, Hafke JB, van Bel AJE, Furch ACU. 2013. Interaction of xylem and phloem during exudation and wound occlusion in Cucurbita maxima. Plant, Cell & Environment 36: 237247.