Role of LONELY GUY genes in indeterminate nodulation on Medicago truncatula
Article first published online: 21 JAN 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 202, Issue 2, pages 582–593, April 2014
How to Cite
Mortier, V., Wasson, A., Jaworek, P., De Keyser, A., Decroos, M., Holsters, M., Tarkowski, P., Mathesius, U. and Goormachtig, S. (2014), Role of LONELY GUY genes in indeterminate nodulation on Medicago truncatula. New Phytologist, 202: 582–593. doi: 10.1111/nph.12681
- Issue published online: 20 MAR 2014
- Article first published online: 21 JAN 2014
- Manuscript Accepted: 11 DEC 2013
- Manuscript Received: 11 SEP 2013
- European Commission Marie Curie International Research Staff Exchange Scheme (IRSES). Grant Number: PIRSES-GA-2008-230830
- Ministerie van de Vlaamse Gemeenschap. Grant Number: CLO/IWT/020714
- Research Foundation-Flanders. Grant Numbers: G.0350.04N, G.0066.07N
- Centre of the Region Haná for the Biotechnological and Agricultural Research. Grant Number: ED 0007/01/01
- Australian Research Council. Grant Number: FT100100669
- LONELY GUY ;
- nodule primordium;
- root growth
- LONELY GUY (LOG) genes encode cytokinin riboside 5′-monophosphate phosphoribohydrolases and are directly involved in the activation of cytokinins.
- To assess whether LOG proteins affect the influence of cytokinin on nodulation, we studied two LOG genes of Medicago truncatula.
- Expression analysis showed that MtLOG1 and MtLOG2 were upregulated during nodulation in a CRE1-dependent manner. Expression was mainly localized in the dividing cells of the nodule primordium. In addition, RNA interference revealed that MtLOG1 is involved in nodule development and that the gene plays a negative role in lateral root development. Ectopic expression of MtLOG1 resulted in a change in cytokinin homeostasis, triggered cytokinin-inducible genes and produced roots with enlarged vascular tissues and shortened primary roots. In addition, those 35S:LOG1 roots also displayed fewer nodules than the wild-type. This inhibition in nodule formation was local, independent of the SUPER NUMERIC NODULES gene, but coincided with an upregulation of the MtCLE13 gene, encoding a CLAVATA3/EMBRYO SURROUNDING REGION peptide.
- In conclusion, we demonstrate that in M. truncatula LOG proteins might be implicated in nodule primordium development and lateral root formation.