Article first published online: 4 FEB 2014
No claim to original European Union works. New Phytologist © 2014 New Phytologist Trust
Volume 202, Issue 3, pages 1069–1082, May 2014
How to Cite
Brouwer, P., Bräutigam, A., Külahoglu, C., Tazelaar, A. O. E., Kurz, S., Nierop, K. G. J., van der Werf, A., Weber, A. P. M. and Schluepmann, H. (2014), Azolla domestication towards a biobased economy?. New Phytologist, 202: 1069–1082. doi: 10.1111/nph.12708
- Issue published online: 10 APR 2014
- Article first published online: 4 FEB 2014
- Manuscript Accepted: 22 DEC 2013
- Manuscript Received: 31 OCT 2013
- AzoFaSt Pathfinder
- European Institute of Technology
- Deutsche Forschungsgemeinschaft. Grant Number: EXC 1028
- Azolla filiculoides ;
- biobased economy;
- sexual reproduction
- Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome.
- Methods for dissemination, cross-fertilization and cryopreservation of the symbiosis Azolla filiculoides–Nostoc azollae are tested based on the fern spores. To study molecular processes in Azolla including spore induction, a database of 37 649 unigenes from RNAseq of microsporocarps, megasporocarps and sporophytes was assembled, then validated.
- Spores obtained year-round germinated in vitro within 26 d. In vitro fertilization rates reached 25%. Cryopreservation permitted storage for at least 7 months. The unigene database entirely covered central metabolism and to a large degree covered cellular processes and regulatory networks. Analysis of genes engaged in transition to sexual reproduction revealed a FLOWERING LOCUS T-like protein in ferns with special features induced in sporulating Azolla fronds.
- Although domestication of a fern–cyanobacteria symbiosis may seem a daunting task, we conclude that the time is ripe and that results generated will serve to more widely access biochemicals in fern biomass for a biobased economy.