A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis

Authors

  • Céline Sorin,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
    2. Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France
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    • These authors contributed equally to this work.
  • Marie Declerck,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
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    • These authors contributed equally to this work.
  • Aurélie Christ,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
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  • Thomas Blein,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
    2. INRA, Institut JP Bourgin, Versailles Cedex, France
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  • Linnan Ma,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
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  • Christine Lelandais-Brière,

    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
    2. Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France
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  • Maria Fransiska Njo,

    1. Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent, Belgium
    2. Department Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium
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  • Tom Beeckman,

    1. Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent, Belgium
    2. Department Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium
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  • Martin Crespi,

    Corresponding author
    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
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  • Caroline Hartmann

    Corresponding author
    1. Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, Gif-sur-Yvette Cedex, France
    2. Université Paris Diderot, Sorbonne Paris Cité, Paris Cedex 13, France
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Summary

  • In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants.
  • By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching.
  • We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels.
  • We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.

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