These authors contributed equally to this work.
A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis
Article first published online: 17 FEB 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 202, Issue 4, pages 1197–1211, June 2014
How to Cite
Sorin, C., Declerck, M., Christ, A., Blein, T., Ma, L., Lelandais-Brière, C., Njo, M. F., Beeckman, T., Crespi, M. and Hartmann, C. (2014), A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. New Phytologist, 202: 1197–1211. doi: 10.1111/nph.12735
- Issue published online: 8 MAY 2014
- Article first published online: 17 FEB 2014
- Manuscript Accepted: 21 JAN 2014
- Manuscript Received: 18 NOV 2013
- Conseil Général de l'Essonne
- French Ministry of Education
- ROOT KBBE
- Interuniversity Attraction Poles Programme. Grant Numbers: IAP VI/33, IUAP P7/29
- Belgian Federal Science Policy Office
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Fig. S1 miR169 sequences and NF-YA family tree.
Fig. S2 MIM169abc have no particular root phenotype.
Fig. S3 The sequestration of miR169defg isoform affects primary root length.
Fig. S4 MIM169defg have smaller cortex cells and fewer cells in other files when compared with WT.
Fig. S5 Expression of NF-YA genes and miR169 in different organs.
Fig. S6 miR169 isoforms accumulation in MIMabc and MIM169defg plants.
Fig. S7 Accumulation of miR169 in 35s:miR169a and 35S:miR169d transgenic lines.
Fig. S8 Root phenotype of over expressing lines (OE) for miR169a and miR169d.
Fig. S9 Diagram of GFP-NF-YA reporter constructs.
Fig. S10 miR169 and miR390b accumulation in agroinfiltrated N. benthamiana leaves.
Fig. S11 35S:NF-YA2 Arabidopsis lines have a dramatic developmental phenotype.
Fig. S12 Root phenotypes of WT and pNF-YA2:HA-NF-YA2wt lines.
Fig. S13 NF-YA2 overexpression does not modify auxin responsive gene expression in a lateral root induction system.
Table S1 Primer sequences for cloning
Table S2 AGI numbers of genes and primers sequences for qPCR and RT