Conserved histidine of metal transporter AtNRAMP1 is crucial for optimal plant growth under manganese deficiency at chilling temperatures
Article first published online: 27 FEB 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 202, Issue 4, pages 1173–1183, June 2014
How to Cite
Ihnatowicz, A., Siwinska, J., Meharg, A. A., Carey, M., Koornneef, M. and Reymond, M. (2014), Conserved histidine of metal transporter AtNRAMP1 is crucial for optimal plant growth under manganese deficiency at chilling temperatures. New Phytologist, 202: 1173–1183. doi: 10.1111/nph.12737
- Issue published online: 8 MAY 2014
- Article first published online: 27 FEB 2014
- Manuscript Accepted: 10 JAN 2014
- Manuscript Received: 3 OCT 2013
- Max Planck Society
- University of Gdansk. Grant Number: 538-M031-B145-13
- chilling temperatures;
- environmental stress;
- metal transporter;
- manganese (Mn) deficiency;
- plant–soil interactions
- Manganese (Mn) is an essential nutrient required for plant growth, in particular in the process of photosynthesis. Plant performance is influenced by various environmental stresses including contrasting temperatures, light or nutrient deficiencies. The molecular responses of plants exposed to such stress factors in combination are largely unknown.
- Screening of 108 Arabidopsis thaliana (Arabidopsis) accessions for reduced photosynthetic performance at chilling temperatures was performed and one accession (Hog) was isolated. Using genetic and molecular approaches, the molecular basis of this particular response to temperature (G × E interaction) was identified.
- Hog showed an induction of a severe leaf chlorosis and impaired growth after transfer to lower temperatures. We demonstrated that this response was dependent on the nutrient content of the soil. Genetic mapping and complementation identified NRAMP1 as the causal gene. Chlorotic phenotype was associated with a histidine to tyrosine (H239Y) substitution in the allele of Hog NRAMP1. This led to lethality when Hog seedlings were directly grown at 4°C.
- Chemical complementation and hydroponic culture experiments showed that Mn deficiency was the major cause of this G × E interaction. For the first time, the NRAMP-specific highly conserved histidine was shown to be crucial for plant performance.