These authors contributed equally to this work.
Complex regulation of secondary metabolism controlling pathogenicity in the phytopathogenic fungus Alternaria alternata
Article first published online: 10 MAR 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 202, Issue 4, pages 1297–1309, June 2014
How to Cite
Takaoka, S., Kurata, M., Harimoto, Y., Hatta, R., Yamamoto, M., Akimitsu, K. and Tsuge, T. (2014), Complex regulation of secondary metabolism controlling pathogenicity in the phytopathogenic fungus Alternaria alternata. New Phytologist, 202: 1297–1309. doi: 10.1111/nph.12754
- Issue published online: 8 MAY 2014
- Article first published online: 10 MAR 2014
- Manuscript Accepted: 3 FEB 2014
- Manuscript Received: 22 NOV 2013
- Grants-in-Aids for Scientific Research (A). Grant Number: 23248007
- Scientific Research (S). Grant Numbers: 19108001, 21228001
- Japanese Society for Promotion of Sciences and Special Coordination Funds
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
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Fig. S1 Transformation-mediated disruption of AKT7.
Fig. S2 AKT7 overexpression strains.
Fig. S3 Cosmid clones encoding host-selective toxin biosynthetic genes of the Japanese pear, strawberry and tangerine pathotypes.
Fig. S4 Alignment of Akt7-1 and fungal proteins from group I of E-class cytochrome P450.
Fig. S5 RT-PCR detection of transcripts of AKT genes.
Fig. S6 Alignment of Akt7-1 and Akt7h.
Fig. S7 Colony growth and morphology of AKT7 disruption and overexpression strains.
Fig. S8 Production of AF-toxin and EDA by an AKT7-1 overexpression strain of the strawberry pathotype.
Fig. S9 Production of ACT-toxin by AKT7-1 overexpression strains of the tangerine pathotype.
Fig. S10 Production of ACT-toxin and EDA by an AKT7-1 overexpression strain of the tangerine pathotype.
Table S1 Alternaria alternata strains used in this study
Table S2 Oligonucleotide primers used in this study
Table S3 Sequences of the 5′ donor splice sites, branch sites and 3′ acceptor splice sites of AKT genes