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Fig. S1 Transformation-mediated disruption of AKT7.

Fig. S2 AKT7 overexpression strains.

Fig. S3 Cosmid clones encoding host-selective toxin biosynthetic genes of the Japanese pear, strawberry and tangerine pathotypes.

Fig. S4 Alignment of Akt7-1 and fungal proteins from group I of E-class cytochrome P450.

Fig. S5 RT-PCR detection of transcripts of AKT genes.

Fig. S6 Alignment of Akt7-1 and Akt7h.

Fig. S7 Colony growth and morphology of AKT7 disruption and overexpression strains.

Fig. S8 Production of AF-toxin and EDA by an AKT7-1 overexpression strain of the strawberry pathotype.

Fig. S9 Production of ACT-toxin by AKT7-1 overexpression strains of the tangerine pathotype.

Fig. S10 Production of ACT-toxin and EDA by an AKT7-1 overexpression strain of the tangerine pathotype.

Table S1 Alternaria alternata strains used in this study

Table S2 Oligonucleotide primers used in this study

Table S3 Sequences of the 5′ donor splice sites, branch sites and 3′ acceptor splice sites of AKT genes