Transcriptomic and lipidomic profiles of glycerolipids during Arabidopsis flower development

Authors

  • Yuki Nakamura,

    Corresponding author
    1. Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan
    2. PRESTO, Japan Science and Technology Agency, Saitama, Japan
    3. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore city, Singapore
    4. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore city, Singapore
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  • Norman Z. W. Teo,

    1. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore city, Singapore
    2. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore city, Singapore
    3. Department of Biological Sciences, National University of Singapore, Singapore city, 117543 Singapore
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  • Guanghou Shui,

    1. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore city, Singapore
    2. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
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  • Christine H. L. Chua,

    1. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore city, Singapore
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  • Wei-Fun Cheong,

    1. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore city, Singapore
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  • Sriram Parameswaran,

    1. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore city, Singapore
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  • Ryota Koizumi,

    1. Department of Biological Sciences, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan
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  • Hiroyuki Ohta,

    1. Center for Biological Resources and Informatics, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan
    2. Earth-Life Science Institute, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan
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  • Markus R. Wenk,

    1. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore city, Singapore
    2. Department of Biological Sciences, National University of Singapore, Singapore city, 117543 Singapore
    3. Swiss Tropical and Public Health Institute, University of Basel, Basel, Switzerland
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  • Toshiro Ito

    Corresponding author
    1. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore city, Singapore
    2. Department of Biological Sciences, National University of Singapore, Singapore city, 117543 Singapore
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Summary

  • Flower glycerolipids are the yet-to-be discovered frontier of the lipidome. Although ample evidence suggests important roles for glycerolipids in flower development, stage-specific lipid profiling in tiny Arabidopsis flowers is challenging. Here, we utilized a transgenic system to synchronize flower development in Arabidopsis.
  • The transgenic plant PAP1::AP1-GR ap1-1 cal-5 showed synchronized flower development upon dexamethasone treatment, which enabled massive harvesting of floral samples of homogenous developmental stages for glycerolipid profiling.
  • Glycerolipid profiling revealed a decrease in concentrations of phospholipids involved in signaling during the early development stages, such as phosphatidic acid and phosphatidylinositol, and a marked increase in concentrations of nonphosphorous galactolipids during the late stage. Moreover, in the midstage, phosphatidylinositol 4,5-bisphosphate concentration was increased transiently, which suggests the stimulation of the phosphoinositide metabolism. Accompanying transcriptomic profiling of relevant glycerolipid metabolic genes revealed simultaneous induction of multiple phosphoinositide biosynthetic genes associated with the increased phosphatidylinositol 4,5-bisphosphate concentration, with a high degree of differential expression patterns for genes encoding other glycerolipid-metabolic genes. The phosphatidic acid phosphatase mutant pah1 pah2 showed flower developmental defect, suggesting a role for phosphatidic acid in flower development.
  • Our concurrent profiling of glycerolipids and relevant metabolic gene expression revealed distinct metabolic pathways stimulated at different stages of flower development in Arabidopsis.

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