Transcriptomic and lipidomic profiles of glycerolipids during Arabidopsis flower development
Article first published online: 28 MAR 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 203, Issue 1, pages 310–322, July 2014
How to Cite
Nakamura, Y., Teo, N. Z. W., Shui, G., Chua, C. H. L., Cheong, W.-F., Parameswaran, S., Koizumi, R., Ohta, H., Wenk, M. R. and Ito, T. (2014), Transcriptomic and lipidomic profiles of glycerolipids during Arabidopsis flower development. New Phytologist, 203: 310–322. doi: 10.1111/nph.12774
- Issue published online: 28 MAY 2014
- Article first published online: 28 MAR 2014
- Manuscript Accepted: 19 FEB 2014
- Manuscript Received: 10 NOV 2013
- Temasek Life Sciences Laboratory
- SystemsX.ch RTD
- Japanese Society for the Promotion of Science (JSPS)
- National Research Foundation of Singapore. Grant Number: NRFCRP001-108
- Japan Science and Technology Agency
- Institute of Plant and Microbial Biology
- Academia Sinica
- Singapore National Research Foundation. Grant Number: 2007-04
- Biomedical Research Council of Singapore. Grant Number: R-183-000-211-305
- National Medical Research Council. Grant Number: R-183-000-224-213
- Arabidopsis thaliana ;
- flower development;
- synchronized flower development
- Flower glycerolipids are the yet-to-be discovered frontier of the lipidome. Although ample evidence suggests important roles for glycerolipids in flower development, stage-specific lipid profiling in tiny Arabidopsis flowers is challenging. Here, we utilized a transgenic system to synchronize flower development in Arabidopsis.
- The transgenic plant PAP1::AP1-GR ap1-1 cal-5 showed synchronized flower development upon dexamethasone treatment, which enabled massive harvesting of floral samples of homogenous developmental stages for glycerolipid profiling.
- Glycerolipid profiling revealed a decrease in concentrations of phospholipids involved in signaling during the early development stages, such as phosphatidic acid and phosphatidylinositol, and a marked increase in concentrations of nonphosphorous galactolipids during the late stage. Moreover, in the midstage, phosphatidylinositol 4,5-bisphosphate concentration was increased transiently, which suggests the stimulation of the phosphoinositide metabolism. Accompanying transcriptomic profiling of relevant glycerolipid metabolic genes revealed simultaneous induction of multiple phosphoinositide biosynthetic genes associated with the increased phosphatidylinositol 4,5-bisphosphate concentration, with a high degree of differential expression patterns for genes encoding other glycerolipid-metabolic genes. The phosphatidic acid phosphatase mutant pah1 pah2 showed flower developmental defect, suggesting a role for phosphatidic acid in flower development.
- Our concurrent profiling of glycerolipids and relevant metabolic gene expression revealed distinct metabolic pathways stimulated at different stages of flower development in Arabidopsis.