These authors contributed equally to the work.
Phosphorylation of transitory starch by α-glucan, water dikinase during starch turnover affects the surface properties and morphology of starch granules
Version of Record online: 3 APR 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 203, Issue 2, pages 495–507, July 2014
How to Cite
Mahlow, S., Hejazi, M., Kuhnert, F., Garz, A., Brust, H., Baumann, O. and Fettke, J. (2014), Phosphorylation of transitory starch by α-glucan, water dikinase during starch turnover affects the surface properties and morphology of starch granules. New Phytologist, 203: 495–507. doi: 10.1111/nph.12801
- Issue online: 19 JUN 2014
- Version of Record online: 3 APR 2014
- Manuscript Accepted: 7 MAR 2014
- Manuscript Received: 27 NOV 2013
- Deutsche Forschungsgemeinschaft. Grant Number: FE 1030/1-1
- 2005. A novel isoform of glucan water dikinase phosphorylates prephosphorylated α-glucans and is involved in starch degradation in Arabidopsis. Plant Journal 41: 595–605. , , , , , .
- 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254. .
- 2013. Starch synthesizing reactions and paths: in vitro and in vivo studies. Journal of Applied Glycoscience 60: 3–20. , , , .
- 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal 16: 735–743. , .
- 2010. A putative phosphatase LSF1 is required for normal starch turnover in Arabidopsis leaves. Plant Physiology 152: 685–697. , , , , , , , , , et al.
- 2012. Control of starch granule numbers in Arabidopsis chloroplasts. Plant Physiology 158: 905–916. , , , , .
- 2013. Starch synthase 4 is essential for coordination of starch granule formation with chloroplast division during Arabidopsis leaf expansion. New Phytologist 200: 1064–1075. , , , , , , , , .
- 2005. Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves. Plant Journal 43: 398–412. , , , , , , , , , et al.
- 1978. The density of wheat starch granules: a trace dilution procedure for determining the density of an immiscible dispersed phase. Starch/Stärke 30: 80–84. , , .
- 2007. Glucan water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases. Plant Physiology 145: 17–28. , , , , , , , , , et al.
- 2004. The glycan substrate of the cytosolic Pho 2 phosphorylase isozyme from Pisum sativum L: identification linkage analysis and subcellular localization. Plant Journal 39: 933–946. , , , , .
- 2005. Identification subcellular localization and biochemical characterization of water-soluble heteroglycans (SHG) in leaves of Arabidopsis thaliana L: distinct SHG reside in the cytosol and in the apoplast. Plant Journal 43: 568–585. , , , , .
- 2012a. Transitory starch and its degradation in higher plant cells. In: Tetlow IJ, ed. Essential reviews in experimental biology: starch: origins, structure and metabolism, vol 5. London, UK: Society for Experimental Biology, 309–372. , , .
- 2012b. Two carbon fluxes to reserve starch in potato (Solanum tuberosum L.) tuber cells are closely interconnected but differently modulated by temperature. Journal of Experimental Botany 63: 3011–3029. , , , , .
- 2011. Glucose-1-phosphate transport into protoplasts and chloroplasts from leaves of Arabidopsis. Plant Physiology 155: 1723–1734. , , , , .
- 2012. Cell-to-cell diversity in a synchronized Chlamydomonas culture as revealed by single-cell analyses. Biophysical Journal 103: 1078–1086. , , , , , .
- 2008. Mass spectrometric quantification of the relative amounts of C6 and C3 position phosphorylated glucosyl residues in starch. Analytical Biochemistry 379: 73–79. , , , , .
- 2008. Glucan water dikinase phosphorylates crystalline maltodextrins and thereby initiates solubilisation. Plant Journal 55: 323–334. , , , , , , , .
- 2010. The Laforin-like dual-specificity phosphatase SEX4 from Arabidopsis hydrolyses both C6- and C3-phosphate esters introduced by starch-related dikinases and thereby affects phase transition of alpha-glucans. Plant Physiology 152: 711–722. , , , , .
- 2009. The two plastidial starch-related dikinases sequentially phosphorylate glucosyl residues at the surface of both the A- and B-allomorph of crystallized maltodextrins but the mode of action differs. Plant Physiology 150: 962–976. , , , .
- 2012a. Starch phosphorylation and dephosphorylation: the consecutive action of starch-related dikinases and phosphatases. In: Tetlow IJ, ed. Essential reviews in experimental biology: starch: origins, structure and metabolism, vol 5. London, UK: Society for Experimental Biology, 279–308. , , .
- 2012b. The plastidial glucan water dikinase GWD catalyses multiple phosphotransfer reactions. FEBS Journal 279: 1953–1966. , , .
- 1970. Studies on starch phosphate. Part 1. Estimation of glucose-6-phosphate residues in starch and the presence of other bound phosphates. Starch/Stärke 22: 338–343. , , .
- 2001. Composition molecular structure and physicochemical properties of tuber and root starches: a review. Carbohydrate Polymers 45: 253–267. .
- 2011. Carbohydrate-active enzymes exemplify entropic principles in metabolism. Molecular System Biology 7: 542. , , , .
- 1999. Cloning and functional analysis of a cDNA encoding a starch synthase from potato Solanum tuberosum L that is predominantly expressed in leaf tissue. Planta 208: 503–511. , , , , .
- 2005. Identification of a novel enzyme required for starch metabolism in Arabidopsis leaves. The phosphoglucan water dikinase. Plant Physiology 137: 242–252. , , , , , .
- 2009. STARCH-EXCESS4 is a laforin-like phosphoglucan phosphatase required for starch degradation in Arabidopsis thaliana. Plant Cell 21: 334–346. , , , , , , , , , et al.
- 2005. Leaf starch degradation comes out of the shadows. Trends in Plant Science 10: 130–137. , , .
- 2014. Double knock-out mutants of Arabidopsis thaliana grown under normal conditions reveal that the plastidial phosphorylase isozyme (PHS1) participates in transitory starch metabolism. Plant Physiology 164: 907–921. , , , , , , , .
- 2011. Starch-related cytosolic heteroglycans in roots from Arabidopsis thaliana. Plant Physiology 168: 1406–1414. , , .
- 2006. Similar protein phosphatases control starch metabolism in plants and glycogen metabolism in mammals. Journal of Biological Chemistry 281: 11 815–11 818. , , , , , , , , , et al.
- 2000. Compartmentation of the starch-related R1 protein in higher plants. Starch/Stärke 52: 179–185. , , , , .
- 2006. Phosphorylation of C6- and C3-positions of glucosyl residues in starch is catalysed by two distinct dikinases. FEBS Letters 580: 4872–4776. , , , , , .
- 2002. The starch-related R1 protein is an alpha-glucan water dikinase. Proceedings of the National Academy of Sciences, USA 99: 7166–7171. , , , , , .
- 2004. Phosphorylation of transitory starch is increased during degradation. Plant Physiology 135: 2068–2077. , , , , .
- 2007. The phenotype of soluble starch synthase IV defective mutants of Arabidopsis thaliana suggests a novel function of elongation enzymes in the control of starch granule formation. Plant Journal 49: 492–504. , , , , , , , , , .
- 2011. The phosphoglucan phosphatase like sex Four2 dephosphorylates starch at the C3-position in Arabidopsis. Plant Cell 23: 4096–4111. , , , , , , , , , et al.
- 2001. The biosynthesis of starch granules. Biomacromolecules 2: 335–341. .
- 1990. Starch degrading enzymes. In: Lea PJ, ed. Methods in plant biochemistry, vol 3. London, UK: Academic Press, 103–128. .
- 2009. Starch granule initiation in Arabidopsis requires the presence of either class IV or class III starch synthases. Plant Cell 21: 2443–2457. , , , , , , , , , et al.
- 1987. Assay of reducing sugars in the nanomole range with 2,2′-bicinchoninate. Analytical Biochemistry 165: 337–340. , .
- 2009. Tape-Arabidopsis Sandwich – a simpler Arabidopsis protoplast isolation method. Plant Methods 5: 16. , , , , , .
- 2001. The Arabidopsis sex1 mutant is defective in the R1 protein a general regulator of starch degradation in plants and not in the chloroplast hexose transporter. Plant Cell 13: 1907–1918. , , , , , , , , , et al.
- 2002. Starch synthesis in Arabidopsis: granule synthesis composition and structure. Plant Physiology 129: 516–529. , , , , , .