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Fig. S1 Protein sequence alignment of Picea glauca major intrinsic proteins (MIPs).

Fig. S2 Neighbor-joining phylogeny of Picea glauca major intrinsic proteins (MIPs).

Fig. S3 Unweighted pair group method with arithmetic average (UPGMA) phylogeny of Picea glauca major intrinsic proteins (MIPs).

Fig. S4 Amino acid multiple sequence alignment of the N-terminal region of Arabidopsis thaliana AtPIP1;3 and the Picea glauca PgPIP1s and of the highly conserved 10 amino acids of the C-terminal region of PIP2s.

Table S1 Gene names, cDNA clone accession numbers, GenBank accession numbers, Picea glauca genome accession numbers and P. abies homolog genome accession numbers

Table S2 Primer sequences used for the gene expression study

Notes S1 Phylogenetic datasets.