The putative Agrobacterium transcriptional activator-like virulence protein VirD5 may target T-complex to prevent the degradation of coat proteins in the plant cell nucleus
Article first published online: 28 MAY 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 203, Issue 4, pages 1266–1281, September 2014
How to Cite
Wang, Y., Peng, W., Zhou, X., Huang, F., Shao, L. and Luo, M. (2014), The putative Agrobacterium transcriptional activator-like virulence protein VirD5 may target T-complex to prevent the degradation of coat proteins in the plant cell nucleus. New Phytologist, 203: 1266–1281. doi: 10.1111/nph.12866
- Issue published online: 31 JUL 2014
- Article first published online: 28 MAY 2014
- Manuscript Accepted: 28 APR 2014
- Manuscript Received: 19 FEB 2014
- Natural Science Foundation of China. Grant Number: 30971748
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Fig. S1 Western blot analysis of the expression of VirD5▵13 in yeast cells.
Fig. S2 Evolutionary relationships of VirD5 homologs and the NLSs on their amino acid sequences.
Fig. S3 Subcellular localization assay for the Os12g0562400.
Fig. S4 Coprecipitation assay for VirD5, AtVIP1 and VirE2 by glutathione sepharose beads.
Fig. S5 Coprecipitation assay for VirD5, AtVIP1 and VBF by glutathione sepharose beads.
Fig. S6 Sequence analysis of virD operon and RT-PCR assays for VirD5, VirD2 and VirE2.
Fig. S7 A model for the putative functions of VirD5.
Table S1 List of oligonucleotides