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Fig. S1 Western blot analysis of the expression of VirD5▵13 in yeast cells.

Fig. S2 Evolutionary relationships of VirD5 homologs and the NLSs on their amino acid sequences.

Fig. S3 Subcellular localization assay for the Os12g0562400.

Fig. S4 Coprecipitation assay for VirD5, AtVIP1 and VirE2 by glutathione sepharose beads.

Fig. S5 Coprecipitation assay for VirD5, AtVIP1 and VBF by glutathione sepharose beads.

Fig. S6 Sequence analysis of virD operon and RT-PCR assays for VirD5, VirD2 and VirE2.

Fig. S7 A model for the putative functions of VirD5.

Table S1 List of oligonucleotides