Root exudation of phytosiderophores from soil-grown wheat
Article first published online: 2 JUN 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 203, Issue 4, pages 1161–1174, September 2014
How to Cite
Oburger, E., Gruber, B., Schindlegger, Y., Schenkeveld, W. D. C., Hann, S., Kraemer, S. M., Wenzel, W. W. and Puschenreiter, M. (2014), Root exudation of phytosiderophores from soil-grown wheat. New Phytologist, 203: 1161–1174. doi: 10.1111/nph.12868
- Issue published online: 31 JUL 2014
- Article first published online: 2 JUN 2014
- Manuscript Accepted: 23 APR 2014
- Manuscript Received: 21 NOV 2013
- Austrian Science Fund. Grant Number: P22798
- 2′-deoxymugineic acid (DMA);
- iron deficiency;
- strategy II;
- trace elements;
- Triticum aestivum cv Tamaro
- For the first time, phytosiderophore (PS) release of wheat (Triticum aestivum cv Tamaro) grown on a calcareous soil was repeatedly and nondestructively sampled using rhizoboxes combined with a recently developed root exudate collecting tool. As in nutrient solution culture, we observed a distinct diurnal release rhythm; however, the measured PS efflux was c. 50 times lower than PS exudation from the same cultivar grown in zero iron (Fe)-hydroponic culture.
- Phytosiderophore rhizosphere soil solution concentrations and PS release of the Tamaro cultivar were soil-dependent, suggesting complex interactions of soil characteristics (salinity, trace metal availability) and the physiological status of the plant and the related regulation (amount and timing) of PS release.
- Our results demonstrate that carbon and energy investment into Fe acquisition under natural growth conditions is significantly smaller than previously derived from zero Fe-hydroponic studies. Based on experimental data, we calculated that during the investigated period (21–47 d after germination), PS release initially exceeded Fe plant uptake 10-fold, but significantly declined after c. 5 wk after germination.
- Phytosiderophore exudation observed under natural growth conditions is a prerequisite for a more accurate and realistic assessment of Fe mobilization processes in the rhizosphere using both experimental and modeling approaches.