Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana
Version of Record online: 28 MAY 2014
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust
Volume 203, Issue 4, pages 1146–1160, September 2014
How to Cite
Lei, L., Li, Y., Wang, Q., Xu, J., Chen, Y., Yang, H. and Ren, D. (2014), Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana. New Phytologist, 203: 1146–1160. doi: 10.1111/nph.12872
- Issue online: 31 JUL 2014
- Version of Record online: 28 MAY 2014
- Manuscript Accepted: 29 APR 2014
- Manuscript Received: 27 MAR 2014
- State Basic Research Program. Grant Number: 2012CB114200
- National Natural Science Foundation of China. Grant Numbers: 31125006, 31030010, 30771124, 31000127
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Fig. S1 Phenotypes of Arabidopsis wild-type (WT), mpk3 and mpk6 seedlings.
Fig. S2 Effect of MKK9 activity on primary root length and lateral root density of Arabidopsis.
Fig. S3 Anthocyanin and Pi (phosphate; H2PO4− and HPO42−) accumulation in Arabidopsis Col wild-type (WT) seedlings under Murashige and Skoog (MS) and low Pi (LP) conditions with or without dexamethasone (DEX).
Fig. S4 Anthocyanin and Pi (phosphate; H2PO4− and HPO42−) contents in Arabidopsis wild-type (WT) and mkk9 seedlings after transfer to Murashige and Skoog (MS) or low Pi (LP) medium.
Fig. S5 Immunoblot analyses of MKK9 mutant transgene expression, and MPK3 and MPK6 kinase activity assays of MKK9 mutant transgenic and crossed Arabidopsis seedlings.
Fig. S6 T-DNA insertion of Arabidopsis WRKY33 and WRKY75 mutants.
Fig. S7 Activation of MKK9-MPK3/MPK6 in Arabidopsis seedlings enhances Pi (phosphate; H2PO4− and HPO42−) accumulation and suppresses anthocyanin production through a camalexin-independent pathway.
Fig. S8 Detection of the interaction between Arabidopsis PHR1 and MPK3 or MPK6, phosphorylation by MPK3 and MPK6 on PHR1, and regulation of PHR1 on MKK9-MPK3/MPK6-enhanced Pi (phosphate; H2PO4− and HPO42−) accumulation.
Table S1 Oligonucleotides used in this study