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Fig. S1 Expression profile of candidate genes.

Fig. S2 SymCRK expression is nodule-specific.

Fig. S3 SymCRK harbors atypical motifs in the DUF26 and kinase domains.

Fig. S4 SymCRK kinase domains are closer to RD-CRK kinases than to nonRD kinases.

Fig. S5 The symCRK endoreduplication index is higher than dnf2.

Fig. S6 Defense-like reactions in dnf2 and symCRK nodules induced by strain Sm2011.

Fig. S7 Phylogenetic tree of DUF26.

Table S1 Candidate genes and corresponding mutant lines

Table S2 Primers used to evaluate genes expression by RT-qPCR

Table S3 Phenotype segregation analysis of the candidate mutant lines

Table S4 Endoreduplication levels of wild-type, dnf2 and symCRK nodule cells