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Fig. S1 FT/TFL1-like genes of Pinus taeda.

Fig. S2 Amino acid sequence alignment of Picea and Pinus FTL1 and FTL2 genes.

Fig. S3 Expression of GI, MYB, PRR1 and FTL2 genes during bud set progression in three populations of Scots pine (Pinus sylvestris).

Fig. S4 Correlation of the mean level of expression of PsGI, PsMYB, PsPRR1 and PsZTL under the different photoperiod treatments with the proportion of bud set in the three populations of Scots pine.

Fig. S5 PsFTL2 and PsGI expression for six time points during the first 48 h in the three studied populations of Scots pine under the different photoperiod treatments.

Fig. S6 Putative duplication event overview of FT/TFL1-like gene in conifers.

Table S1 Primers used to amplify FTL2 and the housekeeping genes in Pinus sylvestris

Table S2 Protein identity matrix of FT, TFL1 and FTLs

Table S3 Nucleotide identity matrix of FT, TFL1 and FTL genes

Table S4 Synonymous sites identity matrix of TFL1 and FTL2 genes

Table S5 Number of non-synonymous substitutions per non-synonymous site (Ka) and number of synonymous substitutions per synonymous site (Ks) between putative PsFTL2 and FTL2 and FTL1 of other species