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Association of CD28 and CTLA-4 gene polymorphisms with aggressive periodontitis in Brazilians

Authors

  • MRMA e Silva,

    1. Laboratory of Cell-Cell Interactions, Department of Morphology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • PR Moreira,

    1. Laboratory of Cell-Cell Interactions, Department of Morphology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • GC da Costa,

    1. Universidade Federal de Viçosa, Belo Horizonte, Brazil
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  • AM Saraiva,

    1. Laboratory of Cell-Cell Interactions, Department of Morphology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • PEA de Souza,

    1. Department of Pathology, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Brazil
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  • SAF Amormino,

    1. Laboratory of Cell-Cell Interactions, Department of Morphology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • JE da Costa,

    1. Department of Clinical, Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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  • KJ Gollob,

    1. Institute for Education and Research, Santa Casa Hospital, Belo Horizonte, Brazil
    2. National Institutes of Science and Technology, Tropical Diseases, INCT-DT, Belo Horizonte, MG, Brazil
    3. Tropical Diseases Research Program, Center for Infections Disease Research, Biosciences Division, SRI International, Melo Park, CA, USA
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  • WO Dutra

    Corresponding author
    1. National Institutes of Science and Technology, Tropical Diseases, INCT-DT, Belo Horizonte, MG, Brazil
    2. Tropical Diseases Research Program, Center for Infections Disease Research, Biosciences Division, SRI International, Melo Park, CA, USA
    • Laboratory of Cell-Cell Interactions, Department of Morphology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
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Correspondence: Walderez Ornelas Dutra, Department of Morphology. Universidade Federal de Minas Gerais-ICB. Av. Antonio Carlos, 6627 Belo Horizonte, MG 31920-000, Brazil. Tel: +55 31 3409 2809, Fax: +55 31 3499 2655, E-mail: waldutra@gmail.com

Abstract

Objective

Susceptibility to and severity of periodontal disease is influenced by gene polymorphisms related to the immune response. Co-stimulatory molecules, such as CD28 and CTLA-4, are critical in the development of such responses. Our hypothesis is that polymorphisms in genes that code for these molecules may be associated with periodontitis. The aim of the study was to investigate the association between +17 (T/C) CD28 and +49 (A/G) CTLA-4 gene polymorphisms and periodontitis in Brazilians.

Materials and methods

Genomic DNA was obtained from oral swabs of 424 individuals categorized into three groups (control group, aggressive, and chronic periodontitis) considering clinical parameters such as probing depth and clinical attachment loss. The genotyping was performed by polymerase chain reaction–restriction fragment length polymorphism.

Results

There was an association between the T genotype of the CD28 polymorphism and aggressive periodontitis (= 0.04). Moreover, the A+ genotype for CTLA-4 was associated with greater clinical attachment loss in non-smokers with aggressive periodontitis (= 0.006, OR = 16.25, CI = 2.25–117.11).

Conclusions

These findings show that T in CD28 + 17 (T/C) and the A+ in CTLA-4 +49 (A/G) genotypes are associated with susceptibility to aggressive periodontal disease. Thus, our study highlights these polymorphisms as potential genetic susceptibility markers of periodontitis in Brazilians.

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