O. Simonetti and G. Lucarini contributed equally to this work.
Microvessel density and VEGF, HIF-1α expression in primary oral melanoma: correlation with prognosis
Article first published online: 27 DEC 2012
© 2012 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 19, Issue 6, pages 620–627, September 2013
How to Cite
Oral Diseases (2013) 19, 620–627
- Issue published online: 10 JUL 2013
- Article first published online: 27 DEC 2012
- Accepted manuscript online: 4 DEC 2012 04:37AM EST
- Manuscript Accepted: 9 NOV 2012
- Manuscript Revised: 23 OCT 2012
- Manuscript Received: 14 SEP 2012
- oral melanoma;
- VEGF ;
- MVD ;
- invasive margin;
To understand the role of angiogenesis and hypoxia in cancer progression of primary oral melanoma (POM).
Materials and Methods
Sixteen malignant primary melanomas were immunostained with markers CD34, VEGF and HIF-1α. Stained cells were counted in the invasive front and inside the tumour, and the differences were compared and correlated with histological parameters and disease-specific survival of the patients.
Tumour invasive front showed increased MVD and increased vessel VEGF and HIF-1α expression compared with the intratumoural compartment. No such differences were seen in tumoural melanocytes of the two compartments. Positive correlations were observed between CD34 and VEGF, CD34 and HIF-1α and VEGF and HIF-1α expression in invasive front vessels. CD34 expression was statistically correlated with the level of infiltration. A significant trend to worse disease-free survival was also determined with increased invasive front vessel expression of CD34, VEGF and HIF-1α.
Our data highlight the importance of the invasive margin in POM biology. The high angiogenic activity and endothelial VEGF and HIF-1α expression in invasive front vessels have a significant impact on patient survival and future agents targeted against VEGF pathway may represent a novel and effective therapeutic opportunity. Larger studies are needed to further address our findings.